BDNF ELISA 试剂盒
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Quick Overview for BDNF ELISA 试剂盒 (ABIN2042357)
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检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 31.2 pg/mL
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 2 pg/mL
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组件
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96-well plate pre-coated with anti-BDNF, Mouse
Lyophilized recombinant BDNF, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Brain-Derived Neurotrophic Factor (BDNF) ELISA Kit
BDNF 适用: 小鼠 AA 129-247 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 大鼠 AA 129-247 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 人, 大鼠, 小鼠, 兔, 马, 山羊 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 兔 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 豚鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 人 AA 129-247 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Mouse BDNF in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kD, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system.1 BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically.2 BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system.3 BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state.4 It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury.5 The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13. 6
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
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别名
- BDNF
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基因ID
- 12064
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UniProt
- P21237
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途径
- RTK signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
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