BDNF ELISA 试剂盒
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北京 101111
Quick Overview for BDNF ELISA 试剂盒 (ABIN411257)
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich Human BDNF ELISA Kit to quantitate Human BDNF in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: H129-R247Capture antibody and Detection antibody: a monoclonal antibody from mouse, a biotinylated monoclonal antibody from mouse
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 15 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Brain-Derived Neurotrophic Factor (BDNF) ELISA Kit
BDNF 适用: 小鼠 AA 129-247 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 大鼠 AA 129-247 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 人, 大鼠, 小鼠, 兔, 马, 山羊 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 豚鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 兔 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 猴 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Brain-Derived Neurotrophic Factor (BDNF) ELISA KitBDNF 适用: 豚鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 7.6%, 4.3%, 4.4%
Inter-Assay Precision (CV%): 8.6%, 5.1%, 5.4% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "Brain-derived neurotrophic factor promotes human granulosa-like tumor cell steroidogenesis and proliferation by activating the FSH receptor-mediated signaling pathway." in: Scientific reports, Vol. 7, Issue 1, pp. 180, (2018) (PubMed).
: "Telomere length and hTERT in mania and subsequent remission." in: Revista brasileira de psiquiatria (Sao Paulo, Brazil : 1999), Vol. 40, Issue 1, pp. 19-25, (2018) (PubMed).
: "Association of serum brain derived neurotropic factor with duration of drug-naive period and positive-negative symptom scores in drug naive schizophrenia." in: PLoS ONE, Vol. 12, Issue 12, pp. e0189373, (2018) (PubMed).
: "Long-term delivery of brain-derived neurotrophic factor (BDNF) from nanoporous silica nanoparticles improves the survival of spiral ganglion neurons in vitro." in: PLoS ONE, Vol. 13, Issue 3, pp. e0194778, (2018) (PubMed).
: "Effect of hyperbaric oxygen on BDNF-release and neuroprotection: Investigations with human mesenchymal stem cells and genetically modified NIH3T3 fibroblasts as putative cell therapeutics." in: PLoS ONE, Vol. 12, Issue 5, pp. e0178182, (2017) (PubMed).
: "Effect of Oxcarbazepine on Serum Brain Derived Neurotrophic Factor in Bipolar Mania: An Exploratory Study." in: Clinical psychopharmacology and neuroscience : the official scientific journal of the Korean College of Neuropsychopharmacology, Vol. 15, Issue 2, pp. 170-176, (2017) (PubMed).
: "Brain-derived neurotrophic factor is increased in serum levels of patients with symptomatic dermographism." in: Postepy dermatologii i alergologii, Vol. 34, Issue 4, pp. 346-349, (2017) (PubMed).
: "Magnetic Beads Enhance Adhesion of NIH 3T3 Fibroblasts: A Proof-of-Principle In Vitro Study for Implant-Mediated Long-Term Drug Delivery to the Inner Ear." in: PLoS ONE, Vol. 11, Issue 2, pp. e0150057, (2016) (PubMed).
: "Neurotherapeutic effect of cord blood derived CD45+ hematopoietic cells in mice after traumatic brain injury." in: Journal of neurotrauma, Vol. 31, Issue 16, pp. 1405-16, (2015) (PubMed).
: "Lithium protects against paraquat neurotoxicity by NRF2 activation and miR-34a inhibition in SH-SY5Y cells." in: Frontiers in cellular neuroscience, Vol. 9, pp. 209, (2015) (PubMed).
: "Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms." in: Neural regeneration research, Vol. 10, Issue 4, pp. 651-8, (2015) (PubMed).
: "Brain-derived neurotrophic factor blood levels are decreased in schizophrenia patients and associate with rs6265 genotypes." in: Clinical biochemistry, Vol. 47, Issue 12, pp. 1052-5, (2014) (PubMed).
: "p75 neurotrophin receptor cleavage by ?- and ?-secretases is required for neurotrophin-mediated proliferation of brain tumor-initiating cells." in: The Journal of biological chemistry, Vol. 289, Issue 12, pp. 8067-85, (2014) (PubMed).
: "Measurement methods of BDNF levels in major depression: a qualitative systematic review of clinical trials." in: The Psychiatric quarterly, Vol. 84, Issue 4, pp. 485-97, (2013) (PubMed).
: "Plasma BDNF and tPA are associated with late-onset geriatric depression." in: Psychiatry and clinical neurosciences, Vol. 64, Issue 3, pp. 249-54, (2010) (PubMed).
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- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
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别名
- BDNF
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背景
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Background: Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8 kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.
Gene Full Name: brain derived neurotrophic factor
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基因ID
- 627
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UniProt
- P23560
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途径
- RTK signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
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