IGFBP5 ELISA 试剂盒
Quick Overview for IGFBP5 ELISA 试剂盒 (ABIN1889356)
抗原
See all IGFBP5 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IGFBP5
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: L20-E271 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: L20-E271
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Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA Kit
IGFBP5 适用: Cow Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 小鼠 Colorimetric Sandwich ELISA 5-3000 pg/mL Cell Culture Supernatant, Plasma, Serum
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 大鼠 AA 20-271 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 犬 Colorimetric Competition ELISA 2.5-50 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA KitIGFBP5 适用: 大鼠, 小鼠 Colorimetric Sandwich ELISA 25-7.000 pg/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Contains 1 IGFBP N-terminal domain.
Tissue Specificity: Most abundant in kidney, uterus and gastrocnemius muscle.
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板类型
- Pre-coated
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实验流程
- mouse IGFBP5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGFBP5 has been precoated onto 96-well plates. Standards(NSO, L20-E271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGFBP5 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse IGFBP5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IGFBP5 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.1, Standard deviation: 0.045, CV(%): 4.1
- Sample 2: n=16, Mean(ng/ml): 3.4, Standard deviation: 0.167, CV(%): 4.9
- Sample 3: n=16, Mean(ng/ml): 6.2, Standard deviation: 0.341, CV(%): 5.5,
- Sample 1: n=24, Mean(ng/ml): 1.5, Standard deviation: 0.084, CV(%): 5.6
- Sample 2: n=24, Mean(ng/ml): 3.8, Standard deviation: 0.247, CV(%): 6.5
- Sample 3: n=24, Mean(ng/ml): 6.7, Standard deviation: 0.476, CV(%): 7.1
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))
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别名
- IGFBP5
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背景
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Protein Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Background: Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The IGFBP5 gene was mapped to chromosome 2q33-q34. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection was inhibitory to growth in 2 human breast cancer cell lines. Stable expression of IGFBP5 in the breast cancer cell lines also inhibited the formation and growth of tumors following injection in athymic mice. IGFBP5 is believed a growth inhibitor and proapoptotic agent in breast cancer cells.
Synonyms: Insulin-like growth factor-binding protein 5,IBP-5,IGF-binding protein 5,IGFBP-5,Igfbp5,Igfbp-5,
Full Gene Name: Insulin-like growth factor-binding protein 5
Cellular Localisation: Secreted. -
基因ID
- 16011
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UniProt
- Q07079
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途径
- WNT signaling, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding
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