我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

CCL3 ELISA 试剂盒

Name: CCL3 ELISA 试剂盒
SKU: ABIN1889299
Availability: OutOfStock

This 小鼠 CCL3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 CCL3 in Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA) 和 Plasma (citrate). This ELISA Kit has been cited in 2+ publications.

ELISA

1 image

(2 references)

产品编号 ABIN1889299

发货至: 中国

Datasheet as PDF

Quick Overview for CCL3 ELISA 试剂盒 (ABIN1889299)

抗原

See all CCL3 ELISA试剂盒

CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

抗原表位

AA 24-92

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

7.8-500 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Discover our top product CCL3 ELISA Kit

最低检测浓度

7.8 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CCL3/MIP1 alpha

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: A24-A92

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<10pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: E.coli
Immunogen sequence: A24-A92
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA Kit
CCL3 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA Kit
CCL3 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA Kit
CCL3 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA Kit
CCL3 适用: 犬 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Plasma, Serum

Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA Kit
CCL3 适用: 大鼠 AA 24-92 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA Kit
CCL3 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA Kit
CCL3 适用: 人 AA 27-92 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA Kit
CCL3 适用: 小鼠 AA 33-262 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA Kit
CCL3 适用: 兔 Colorimetric 7.8-500 pg/mL Plasma, Serum, Tissue Homogenate

Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA Kit
CCL3 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Tissue Specificity: Expressed in lung, spleen, and pancreas.

板类型

Pre-coated

实验流程

mouse CCL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL3 has been precoated onto 96-well plates. Standards(E.coli, A24-A92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL3 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse CCL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse CCL3 standard solution and each sample is measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 102, Standard deviation: 3.88, CV(%): 3.8
- Sample 2: n=16, Mean(pg/ml): 234, Standard deviation: 9.6, CV(%): 4.1
- Sample 3: n=16, Mean(pg/ml): 421, Standard deviation: 22.3, CV(%): 5.3,
- Sample 1: n=24, Mean(pg/ml): 86, Standard deviation: 4.82, CV(%): 5.6
- Sample 2: n=24, Mean(pg/ml): 255, Standard deviation: 17, CV(%): 6.7
- Sample 3: n=24, Mean(pg/ml): 437, Standard deviation: 31, CV(%): 7.1
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Du, Chen, Wang, Sun: "Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha." in: Journal of ethnopharmacology, Vol. 214, pp. 141-152, (2018) (PubMed).

Aziz, Cui, Das, Brown, Ardell, Febbraio, Pluskota, Han, Wu, Ballantyne, Smith, Cathcart, Yakubenko: "The Upregulation of Integrin αDβ2 (CD11d/CD18) on Inflammatory Macrophages Promotes Macrophage Retention in Vascular Lesions and Development of Atherosclerosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 198, Issue 12, pp. 4855-4867, (2017) (PubMed).
抗原 See all CCL3 ELISA试剂盒

CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

别名

CCL3

背景

Protein Function: Monokine with inflammatory, pyrogenic and chemokinetic properties. Has a potent chemotactic activity for eosinophils. Binding to a high-affinity receptor activates calcium release in neutrophils.
Background: Chemokine(C-C motif) ligand 3(CCL3), so known as Macrophage inflammatory protein-1alpha(MIP-1 alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 directly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.
Synonyms: C-C motif chemokine 3,Heparin-binding chemotaxis protein,L2G25B,Macrophage inflammatory protein 1-alpha,MIP-1-alpha,SIS-alpha,Small-inducible cytokine A3,TY-5,Ccl3,Mip1a, Scya3,
Full Gene Name: C-C motif chemokine 3
Cellular Localisation: Secreted.

基因ID

20302

UniProt

P10855

途径

Cellular Response to Molecule of Bacterial Origin, Autophagy