CCL3 ELISA 试剂盒
(2 references)
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Quick Overview for CCL3 ELISA 试剂盒 (ABIN6720004)
抗原
See all CCL3 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 7.8 pg/mL
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Supplier Product No.
- koa0258
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Supplier
- Rockland
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原理
- Mouse CCL3 - MIP1 alpha Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: A24-A92 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA Kit
CCL3 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 适用: 人 Colorimetric Sandwich ELISA 23.438 pg/mL - 1500 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 适用: 犬 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Plasma, Serum
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 适用: 小鼠 AA 24-92 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 适用: 大鼠 AA 24-92 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 适用: 大鼠 Colorimetric Sandwich ELISA 16-1000 pg/mL Cell Lysate, Plasma, Serum
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说明
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Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse CCL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. We recommend that each mouse CCL3 standard solution and each sample is measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 4.1%, 5.4%, 4%
Inter-Assay Precision (CV%): 4.2%, 7.2%, 5.8% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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: "Immuno-protective vesicle-crosslinked hydrogel for allogenic transplantation." in: Nature communications, Vol. 15, Issue 1, pp. 5176, (2024) (PubMed).
: "Understanding pathogen-host interplay by expression profiles of lncRNA and mRNA in the liver of Echinococcus multilocularis-infected mice." in: PLoS neglected tropical diseases, Vol. 16, Issue 5, pp. e0010435, (2022) (PubMed).
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- CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
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别名
- CCL3/MIP1 alpha
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背景
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Background: Chemokine (C-C motif) ligand 3 (CCL3), so known as Macrophage inflammatory protein-1alpha (MIP-1 alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 ly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.
Gene Full Name: C-C motif chemokine ligand 3
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基因ID
- 20302
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NCBI登录号
- NP_035467
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UniProt
- P10855
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途径
- Cellular Response to Molecule of Bacterial Origin, Autophagy
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