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FLT3LG ELISA 试剂盒

Name: FLT3LG ELISA 试剂盒
SKU: ABIN1889292
Availability: OutOfStock

This 小鼠 FLT3LG ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 FLT3LG. This ELISA Kit has been cited in1 publication.

ELISA

1 image

(1 reference)

产品编号 ABIN1889292

发货至: 中国

Quick Overview for FLT3LG ELISA 试剂盒 (ABIN1889292)

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See all FLT3LG ELISA试剂盒

FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

抗原表位

AA 27-188

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2-2000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum

Discover our top product FLT3LG ELISA Kit

最低检测浓度

31.2 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Flt-3ligand

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: NSO
Immunogen sequence: G27-R188

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<10pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: NSO
Immunogen sequence: G27-R188
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FLT3LG 适用: 小鼠 Colorimetric Sandwich ELISA 15-5000 pg/mL Cell Culture Supernatant, Plasma, Serum

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

板类型

Pre-coated

实验流程

mouse Flt-3ligand ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Flt-3ligand has been precoated onto 96-well plates. Standards(NSO, G27-R188) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Flt-3ligand is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Flt-3ligand amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse Flt-3ligand standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Flt-3ligand standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 207, Standard deviation: 11.18, CV(%): 5.4
- Sample 2: n=16, Mean(pg/ml): 556, Standard deviation: 30.02, CV(%): 5.4
- Sample 3: n=16, Mean(pg/ml): 1324, Standard deviation: 59.58, CV(%): 4.5,
- Sample 1: n=24, Mean(pg/ml): 212, Standard deviation: 17.81, CV(%): 8.4
- Sample 2: n=24, Mean(pg/ml): 545, Standard deviation: 36.52, CV(%): 6.7
- Sample 3: n=24, Mean(pg/ml): 1318, Standard deviation: 86.99, CV(%): 6.6
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Chen, Zhao, Wu, Zou, Luo, Li, Xie, Liang: "The role of RIP1 and RIP3 in the development of aplastic anemia induced by cyclophosphamide and busulphan in mice." in: International journal of clinical and experimental pathology, Vol. 7, Issue 12, pp. 8411-20, (2015) (PubMed).
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FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

别名

FLT3LG

背景

Protein Function: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
Background: Fms-related tyrosine kinase 3 ligand(FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor(SCF) and colony stimulating factor 1(CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells(DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.
Synonyms: Fms-related tyrosine kinase 3 ligand,Flt3 ligand,Flt3L,SL cytokine,Flt3lg,Flt3l,
Full Gene Name: Fms-related tyrosine kinase 3 ligand
Cellular Localisation: Cell membrane, Single-pass type I membrane protein.

基因ID

14256

UniProt

P49772

途径

RTK signaling