DKK1 ELISA 试剂盒
(1 reference)Quick Overview for DKK1 ELISA 试剂盒 (ABIN1672840)
抗原
See all DKK1 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 46.9 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse DKK1
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: CHO
Immunogen sequence: S30-H272 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <15pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: CHO
Immunogen sequence: S30-H272
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Dickkopf Homolog 1 (DKK1) ELISA Kit
DKK1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 人 AA 32-266 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 大鼠 AA 23-270 Colorimetric Sandwich ELISA 46.9-3000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 小鼠 Colorimetric Sandwich ELISA 25-6000 pg/mL Cell Culture Supernatant, Plasma, Serum
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 小鼠 Colorimetric Sandwich ELISA 46.9 pg/mL - 3000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- mouse DKK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for DKK1 has been precoated onto 96-well plates. Standards(CHO, S30-H272) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DKK1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse DKK1 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 375.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL mouse DKK1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse DKK1 standard solution and each sample is measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 228, Standard deviation: 12.54, CV(%): 5.5
- Sample 2: n=16, Mean(pg/ml): 845, Standard deviation: 56.6, CV(%): 6.7
- Sample 3: n=16, Mean(pg/ml): 1474, Standard deviation: 91.39, CV(%): 6.2,
- Sample 1: n=24, Mean(pg/ml): 259, Standard deviation: 17.1, CV(%): 6.6
- Sample 2: n=24, Mean(pg/ml): 897, Standard deviation: 66.4, CV(%): 7.4
- Sample 3: n=24, Mean(pg/ml): 1532, Standard deviation: 105.7, CV(%): 6.9
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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: "SIRT6 deficiency culminates in low-turnover osteopenia." in: Bone, Vol. 81, pp. 168-77, (2015) (PubMed).
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: "SIRT6 deficiency culminates in low-turnover osteopenia." in: Bone, Vol. 81, pp. 168-77, (2015) (PubMed).
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- DKK1 (Dickkopf Homolog 1 (DKK1))
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别名
- DKK1
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背景
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Protein Function: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. .
Background: Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
Synonyms: Dickkopf-related protein 1,Dickkopf-1,Dkk-1,mDkk-1,Dkk1,
Full Gene Name: Dickkopf-related protein 1
Cellular Localisation: Secreted. -
基因ID
- 13380
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UniProt
- O54908
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途径
- WNT signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation
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