DKK1 ELISA 试剂盒
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- 抗原 See all DKK1 ELISA试剂盒
- DKK1 (Dickkopf Homolog 1 (DKK1))
- 抗原表位
- AA 32-266
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 62.5-4000 pg/mL
- 最低检测浓度
- 62.5 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human DKK-1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: sf21
Immunogen sequence: T32-H266 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: sf21
Immunogen sequence: T32-H266 - Featured
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- Discover our top product DKK1 ELISA Kit
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Dickkopf Homolog 1 (DKK1) ELISA Kit
DKK1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 小鼠 AA 30-272 Colorimetric Sandwich ELISA 46.9-3000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 大鼠 AA 23-270 Colorimetric Sandwich ELISA 46.9-3000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 小鼠 Colorimetric Sandwich ELISA 46.9 pg/mL - 3000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 板类型
- Pre-coated
- 实验流程
- human DKK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DKK1 has been precoated onto 96-well plates. Standards(sf21,T32-H266) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DKK1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DKK1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human DKK1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human DKK1 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 610, Standard deviation: 32.94, CV(%): 5.4
- Sample 2: n=16, Mean(pg/ml): 1503, Standard deviation: 73.65, CV(%): 4.9
- Sample 3: n=16, Mean(pg/ml): 2715, Standard deviation: 103.2, CV(%): 3.8,
- Sample 1: n=24, Mean(pg/ml): 723, Standard deviation: 59.3, CV(%): 8.2
- Sample 2: n=24, Mean(pg/ml): 1862, Standard deviation: 126.6, CV(%): 6.8
- Sample 3: n=24, Mean(pg/ml): 3035, Standard deviation: 169.96, CV(%): 5.6
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all DKK1 ELISA试剂盒
- DKK1 (Dickkopf Homolog 1 (DKK1))
- 别名
- DKK1 (DKK1 产品)
- 别名
- DKK-1 ELISA Kit, SK ELISA Kit, AI414495 ELISA Kit, SGY ELISA Kit, SGY-1 ELISA Kit, SGY1 ELISA Kit, Soggy ELISA Kit, mSgy ELISA Kit, dkk1 ELISA Kit, dkk ELISA Kit, dkk-1 ELISA Kit, xdkk1 ELISA Kit, Xdkk-1 ELISA Kit, Dickkopf1 ELISA Kit, dickkopf-1 ELISA Kit, mdkk-1 ELISA Kit, dkk1-A ELISA Kit, dickkopf WNT signaling pathway inhibitor 1 ELISA Kit, dickkopf like acrosomal protein 1 ELISA Kit, dickkopf-related protein 1 ELISA Kit, dickkopf-like 1 ELISA Kit, dickkopf WNT signaling pathway inhibitor 1b ELISA Kit, dickkopf protein ELISA Kit, dickkopf WNT signaling pathway inhibitor 1 S homeolog ELISA Kit, DKK1 ELISA Kit, Dkk1 ELISA Kit, DKKL1 ELISA Kit, LOC100228789 ELISA Kit, Dkkl1 ELISA Kit, dkk1b ELISA Kit, dkk1 ELISA Kit, dickkopf ELISA Kit, dkk1.S ELISA Kit
- 背景
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Background: Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
Synonyms: Dickkopf-like protein 1 ,DKK1 ,
Full Gene Name: dickkopf WNT signaling pathway inhibitor 1
- 基因ID
- 22943
- UniProt
- I1W660
- 途径
- WNT signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation
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