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Galectin 3 ELISA 试剂盒

Name: Galectin 3 ELISA 试剂盒
SKU: ABIN1672793
Availability: OutOfStock

This 小鼠 Galectin 3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 Galectin 3.

ELISA

1 image

产品编号 ABIN1672793

发货至: 中国

Quick Overview for Galectin 3 ELISA 试剂盒 (ABIN1672793)

抗原

See all Galectin 3 (LGALS3) ELISA试剂盒

Galectin 3 (LGALS3)

抗原表位

AA 2-264

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5-4000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Discover our top product LGALS3 ELISA Kit

最低检测浓度

62.5 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Galectin-3/LGALS3

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: A2-I264

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<10pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: E.coli
Immunogen sequence: A2-I264
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LGALS3 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

板类型

Pre-coated

实验流程

mouse Galectin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Galectin-3 has been precoated onto 96-well plates. Standards(E.coli, A2-I264) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Galectin-3 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse Galectin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Galectin-3 standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 850, Standard deviation: 37.4, CV(%): 4.4
- Sample 2: n=16, Mean(pg/ml): 1347, Standard deviation: 66, CV(%): 4.9
- Sample 3: n=16, Mean(pg/ml): 2932, Standard deviation: 161.3, CV(%): 5.5,
- Sample 1: n=24, Mean(pg/ml): 795, Standard deviation: 54.06, CV(%): 6.8
- Sample 2: n=24, Mean(pg/ml): 1046, Standard deviation: 99.83, CV(%): 7.1
- Sample 3: n=24, Mean(pg/ml): 2758, Standard deviation: 201.3, CV(%): 7.3
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
抗原 See all Galectin 3 (LGALS3) ELISA试剂盒

Galectin 3 (LGALS3)

别名

LGALS3

物质类

Chemical

背景

Background: Galectin-3(GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21â€"q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.
Synonyms: Galectin ,Lgals3 ,mCG_6908 ,
Full Gene Name: Galectin

基因ID

16854

UniProt

Q8C253

途径

RTK signaling