c-MET ELISA 试剂盒
Quick Overview for c-MET ELISA 试剂盒 (ABIN1672789)
抗原
See all c-MET (MET) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 62.5 pg/mL
-
原理
- For quantitative detection of mouse C-MET in cell culture supernates, serum and plasma(heparin, EDTA).
-
品牌
- PicoKine™
-
Analytical Method
- Quantitative
-
特异性
- Natural and recombinant mouse C-MET
-
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- <5pg/mL
-
组件
-
- 96-well plate precoated with anti- mouse C-MET antibod - 1
- Lyophilized recombinant mouse C-MET standar - 10ng/tubex2
- Biotinylated anti- mouse C-MET antibod - 130μl(dilution 1:100)
- Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
- Sample diluent buffe - 30 ml
- Antibody diluent buffe - 12ml
- ABC diluent buffe - 12ml
- TMB color developing agen - 10ml
- TMB stop solutio - 10ml
-
试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
-
免疫原
-
Expression system for standard: sf21
Immunogen sequence: E25-R306(alpha)&S307-N929(beta)
-
-
Met Proto-Oncogene (MET) ELISA Kit
MET 适用: 人, 小鼠, 大鼠 pTyr1234 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Met Proto-Oncogene (MET) IQ-ELISA KitMET 适用: 人 qPCR Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
Met Proto-Oncogene (MET) ELISA KitMET 适用: 人, 小鼠, 大鼠 pTyr1234 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Met Proto-Oncogene (MET) ELISA KitMET 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Met Proto-Oncogene (MET) ELISA KitMET 适用: 人 AA 25-307 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Met Proto-Oncogene (MET) ELISA KitMET 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
-
-
应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
-
板类型
- Pre-coated
-
实验流程
- mouse C-MET ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for C-MET has been precoated onto 96-well plates. Standards(sf21, E25-R306(α) &S307-N929(β)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for C-MET is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse C-MET amount of sample captured in plate.
-
实验流程
-
Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse C-MET standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse C-MET standard solution and each sample be measured in duplicate.
-
实验精密度
-
- Sample 1: n=16, Mean(ng/ml): 0.51, Standard deviation: 0.022, CV(%): 4.3
- Sample 2: n=16, Mean(ng/ml): 1.7, Standard deviation: 0.092, CV(%): 5.4
- Sample 3: n=16, Mean(ng/ml): 2.8, Standard deviation: 0.146, CV(%): 5.2,
- Sample 1: n=24, Mean(ng/ml): 0.57, Standard deviation: 0.029, CV(%): 5.1
- Sample 2: n=24, Mean(ng/ml): 1.8, Standard deviation: 0.137, CV(%): 7.6
- Sample 3: n=24, Mean(ng/ml): 3.2, Standard deviation: 0.221, CV(%): 6.9
-
限制
- 仅限研究用
-
-
-
注意事项
- Avoid multiple freeze-thaw cycles.
-
储存条件
- -20 °C,4 °C
-
储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
-
有效期
- 12 months
-
-
- c-MET (MET) (Met Proto-Oncogene (MET))
-
别名
- MET
-
背景
- C-Met(MET or MNNG HOS Transforming gene) is a proto-oncogene that encodes a protein known as hepatocyte growth factor receptor(HGFR). MET proto-oncogene has a total length of 125,982 bp, and it is located in the 7q31 locus of chromosome 7. MET is a membrane receptor that is essential for embryonic development and wound healing. Activation of MET triggers mitogenesis, and morphogenesis.
-
基因ID
- 17295
-
UniProt
- A4FUV6
-
途径
- RTK signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
抗原 See all c-MET (MET) ELISA试剂盒
-
