Cardiotrophin 1 ELISA 试剂盒
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- 抗原 See all Cardiotrophin 1 (CTF1) ELISA试剂盒
- Cardiotrophin 1 (CTF1)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- For quantitative detection of CT-1 in human serum, body fluids, tissue lysates or cell culture supernatants.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 灵敏度
- < 10 pg/mL
- 组件
- 1. One 96-well plate pre-coated with anti-Human CT-1 antibody 2. Lyophilized Human CT-1 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Human CT-1 antibody (Concentrated): 130 µl.
- 试剂未包括
- 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L
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- Discover our best selling CTF1 ELISA Kit
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- Discover our top product CTF1 ELISA Kit
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Cardiotrophin 1 (CTF1) ELISA Kit
CTF1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 小鼠 Colorimetric Sandwich ELISA 0.2-60 ng/mL Cell Culture Supernatant, Plasma, Serum
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 人 AA 2-201 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 小鼠 AA 2-203 Colorimetric Sandwich ELISA
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 说明
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This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CT-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CT-1 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the CT-1 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of CT-1 can be calculated.
- 板类型
- Pre-coated
- 试剂准备
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- Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.
- 样品制备
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Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Cell culture supernatants, tissue lysate or body fluids: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C . Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP. - 限制
- 仅限研究用
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- 储存液
- Sodium azide, Thimerosal (Merthiolate)
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- 抗原 See all Cardiotrophin 1 (CTF1) ELISA试剂盒
- Cardiotrophin 1 (CTF1)
- 别名
- Cardiotrophin-1 / CT-1 (CTF1 产品)
- 别名
- CTF1 ELISA Kit, ct1 ELISA Kit, ct-1 ELISA Kit, ctf2 ELISA Kit, CT-1 ELISA Kit, CT1 ELISA Kit, cardiotrophin 1 ELISA Kit, cardiotrophin-1 ELISA Kit, CTF1 ELISA Kit, ctf1 ELISA Kit, Ctf1 ELISA Kit, LOC100736818 ELISA Kit
- 背景
- Cardiotrophin-1 (CT-1) is a cytokine. CTF1 gene contains 3 exons, spans over 6 to 7 kb. It is a cardiac hypertrophic factor of 21.5 kDa and a protein member of the IL-6 cytokine family. It is highly expressed in the heart, skeletal muscle, prostate and ovary and to lower levels in lung, kidney, pancreas, thymus, testis and small intestine. CT-1 is associated with the pathophysiology of heart diseases, including hypertension, myocardial infarction, valvular heart disease, and congestive heart failure. This protein exerts its cellular effects by interacting with the glycoprotein 130 (gp130)/leukemia inhibitory factor receptor beta (LIFR) heterodimer.
- 途径
- JAK/STAT Signaling
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