Cardiotrophin 1 ELISA 试剂盒
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- 抗原 See all Cardiotrophin 1 (CTF1) ELISA试剂盒
- Cardiotrophin 1 (CTF1)
- 抗原表位
- AA 2-201
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 156-10000 pg/mL
- 最低检测浓度
- 156 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Cardiotrophin-1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: S2-A201 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: S2-A201 - Featured
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Cardiotrophin 1 (CTF1) ELISA Kit
CTF1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 人 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 小鼠 Colorimetric Sandwich ELISA 0.2-60 ng/mL Cell Culture Supernatant, Plasma, Serum
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 小鼠 AA 2-203 Colorimetric Sandwich ELISA
Cardiotrophin 1 (CTF1) ELISA KitCTF1 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Highly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes.
- 板类型
- Pre-coated
- 实验流程
- human CT-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CT-1 has been precoated onto 96-well plates. Standards(E.coli, S2-A201) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CT-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CT-1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CT-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CT-1 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 253, Standard deviation: 11.38, CV(%): 4.5
- Sample 2: n=16, Mean(pg/ml): 759, Standard deviation: 35.67, CV(%): 4.7
- Sample 3: n=16, Mean(pg/ml): 2042, Standard deviation: 98.09, CV(%): 4.9,
- Sample 1: n=24, Mean(pg/ml): 264, Standard deviation: 12.14, CV(%): 4.6
- Sample 2: n=24, Mean(pg/ml): 788, Standard deviation: 37.82, CV(%): 4.8
- Sample 3: n=24, Mean(pg/ml): 1946, Standard deviation: 97.30, CV(%): 5.0
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all Cardiotrophin 1 (CTF1) ELISA试剂盒
- Cardiotrophin 1 (CTF1)
- 别名
- CTF1 (CTF1 产品)
- 别名
- CTF1 ELISA Kit, ct1 ELISA Kit, ct-1 ELISA Kit, ctf2 ELISA Kit, CT-1 ELISA Kit, CT1 ELISA Kit, cardiotrophin 1 ELISA Kit, cardiotrophin-1 ELISA Kit, CTF1 ELISA Kit, ctf1 ELISA Kit, Ctf1 ELISA Kit, LOC100736818 ELISA Kit
- 背景
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Protein Function: Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.
Background: Cardiotrophin-1(CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor(LIF), ciliary neurotrophic factor(CNTF), oncostatin M(OSM), interleukin-6(IL6), and interleukin-11(IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80 % amino acid identity with the 203-amino acid mouse CT-1 sequence, however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidney.
Synonyms: Cardiotrophin-1,CT-1,CTF1,
Full Gene Name: Cardiotrophin-1
Cellular Localisation: Secreted. - 基因ID
- 1489
- UniProt
- Q16619
- 途径
- JAK/STAT Signaling
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