His Tag 抗体
(His Tag)
His Tag Antibodies are commonly used in molecular biology and protein research. The His tag, which consists of a short sequence of histidine amino acids, is often added to recombinant proteins during their expression in host cells. This tag allows for easy purification and detection of the protein of interest using affinity chromatography and antibody-based assays.
Here are some common applications of antibodies that detect His tags:
- Protein Purification: His-tagged proteins can be purified using a technique called immobilized metal affinity chromatography (IMAC). In this method, a column containing metal ions (usually nickel or cobalt) is used to capture His-tagged proteins, which bind to the metal ions through the histidine residues. Antibodies that recognize the His tag can also be used in the purification process to ensure the specificity of the captured protein.
- Protein Detection: Antibodies that specifically bind to His tags are used in techniques such as Western blotting and ELISA to detect the presence of His-tagged proteins in a sample. The antibody is typically conjugated with a detectable marker (such as an enzyme or a fluorescent molecule) that allows visualization and quantification of the target protein.
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209 results :
Rockland Original
Rockland 200-301-382
WB, ELISA, IP
宿主: 小鼠
Monoclonal
33D10-D2-G8
unconjugated
4 references
Rockland Original
Rockland 200-304-382
WB, ELISA, FACS, DB, FM, FLISA
宿主: 小鼠
Monoclonal
33D10-D2-G8
Cy3
3 references
Rockland Original
Rockland 200-345-382
WB, ELISA, DB, FM, FLISA
宿主: 小鼠
Monoclonal
33D10-D2-G8
DyLight 800
1 reference
Rockland Original
Rockland 200-343-382
WB, ELISA, DB, FM, FLISA
宿主: 小鼠
Monoclonal
33D10-D2-G8
DyLight 649
5 references
Rockland Original
Rockland 200-303-382
WB, ELISA, IHC
宿主: 小鼠
Monoclonal
33D10-D2-G8
HRP
1 reference
Rockland Original
Rockland 200-310-382
FACS, DB, FM, FLISA
宿主: 小鼠
Monoclonal
33D10-D2-G8
Cy5
Rockland Original
Rockland 200-306-382
WB, ELISA, IHC
宿主: 小鼠
Monoclonal
33D10-D2-G8
Biotin
1 reference
Rockland Original
Rockland 200-301-b90
WB, ELISA, IHC
宿主: 小鼠
Monoclonal
33D10-D2-G8
unconjugated
Rockland Original
适用: Various Species
ELISA, IP, ICC, IHC (fro), CLIA
宿主: 小鼠
Monoclonal
D10
unconjugated
1 image
Latest Publications for our His Tag 抗体
: "Insecticidal activities of three recombinant venom proteins of the predatory stink bug, Arma custos." in: Pest management science, Vol. 80, Issue 12, pp. 6473-6482, (2024) (PubMed).: "Reverse-Phase Protein Microarrays for Overexpressed Escherichia coli Lysates Reveal a Novel Tyrosine Kinase." in: Analytical chemistry, Vol. 96, Issue 21, pp. 8721-8729, (2024) (PubMed).
: "Multispecies biofilm architecture determines bacterial exposure to phages." in: PLoS biology, Vol. 20, Issue 12, pp. e3001913, (2022) (PubMed).
: "Development and applications of sialoglycan-recognizing probes (SGRPs) with defined specificities: exploring the dynamic mammalian sialoglycome." in: Glycobiology, Vol. 32, Issue 12, pp. 1116-1136, (2022) (PubMed).
: "Sialoglycan-binding patterns of bacterial AB5 toxin B subunits correlate with host range and toxicity, indicating evolution independent of A subunits." in: The Journal of biological chemistry, Vol. 298, Issue 5, pp. 101900, (2022) (PubMed).
: "Yeast-produced fructosamine-3-kinase retains mobility after ex vivo intravitreal injection in human and bovine eyes as determined by Fluorescence Correlation Spectroscopy." in: International journal of pharmaceutics, Vol. 621, pp. 121772, (2022) (PubMed).
: "ADAMTS4-specific MR probe to assess aortic aneurysms in vivo using synthetic peptide libraries." in: Nature communications, Vol. 13, Issue 1, pp. 2867, (2022) (PubMed).
: "Thiol-based functional mimicry of phosphorylation of the two-component system response regulator ArcA promotes pathogenesis in enteric pathogens." in: Cell reports, Vol. 37, Issue 12, pp. 110147, (2022) (PubMed).
: "Identification of a Thyroid Hormone Binding Site in Hsp90 with Implications for Its Interaction with Thyroid Hormone Receptor Beta." in: ACS omega, Vol. 7, Issue 33, pp. 28932-28945, (2022) (PubMed).
: "Dual Bcl-XL /Bcl-2 inhibitors discovered from DNA-encoded libraries using a fragment pairing strategy." in: Bioorganic & medicinal chemistry, Vol. 44, pp. 116282, (2021) (PubMed).
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