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CREB1 抗体 (pSer133)

This anti-CREB1 antibody is a 兔 多克隆 antibody detecting CREB1 in WB, IHC 和 ELISA. Suitable for 人, 小鼠 和 大鼠. This Primary Antibody has been cited in 1 publication.
Rockland
产品编号 ABIN97863
Supplier Product No.: 600-401-270
发货至: 中国

Quick Overview for CREB1 抗体 (pSer133) (ABIN97863)

抗原

See all CREB1 抗体
CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

适用

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克隆类型

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多克隆

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This CREB1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA
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    pSer133

    Supplier Product No.

    600-401-270

    Supplier

    Rockland

    原理

    CREB phospho S133 Antibody

    产品特性

    Synonyms: rabbit anti-CREB pS133 Antibody, Cyclic AMP-responsive element-binding protein 1, Active transcription factor CREB antibody, cAMP responsive element binding protein 1 antibody, CREB 1 antibody, CREB-1, CREB1 antibody, MGC9284 antibody, Transactivator protein antibody

    纯化方法

    Anti-CREB pS133 was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first sequentially pre-adsorbed against a control E.

    过滤

    Sterile filtered

    免疫原

    Immunogen: CREB phospho peptide corresponding to amino acid residues 122-147 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

    Immunogen Type: Conjugated Peptide

    亚型

    IgG
  • 应用备注

    Immunohistochemistry Dilution: 20 μg/mL

    Application Note: Anti-CREB pS133 antibody reacts with phosphorylated human CREB at pS133 and shows minimal reactivity by western blot with non-phosphorylated CREB and minimal reactivity (1 % ) by ELISA against the non-phosphorylated form of the immunizing peptide.  This antibody was assayed against a variety of tissues including fibroblasts and B-cell lysates.  Bands of 46 and 43 kDa corresponding to phosphorylated CREB are observed in western blots.  Anti-CREB pS133 is suitable for the detection by immunoblot of phosphorylated human, mouse and rat CREB.  No cross-reactivity occurs with non-phosphorylated CREB.  For immunohistochemistry, formalin fixed, paraffin embedded human tissue shows moderate to strong nuclear staining in a variety of cells with minimal background staining.  Although not tested this antibody is likely functional by FACS and immunoprecipitation.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:1,000 - 1:6,000

    Other: User Optimized

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.064 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • Philp, Chen, Lan, Meyer, Murphy, Knapp, Olfert, McCurdy, Marcotte, Hogan, Baar, Schenk et al.: "Sirtuin 1 (SIRT1) deacetylase activity is not required for mitochondrial biogenesis or peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) deacetylation following ..." in: The Journal of biological chemistry, Vol. 286, Issue 35, pp. 30561-70, (2011) (PubMed).

  • 抗原

    CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

    别名

    CREB1

    背景

    Background: The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMP-dependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II.  Alternate splicing of this gene results in two transcript variants encoding different isoforms.

    基因ID

    1385

    UniProt

    P16220

    途径

    TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation
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