CREB1 抗体 (pSer133)
(1 reference)
-
- 抗原 See all CREB1 抗体
- CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))
-
抗原表位
- pSer133
-
适用
- 人, 小鼠, 大鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This CREB1 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Supplier Product No.
- 600-401-270
- Supplier
- Rockland
- 原理
- CREB phospho S133 Antibody
- 产品特性
- Synonyms: rabbit anti-CREB pS133 Antibody, Cyclic AMP-responsive element-binding protein 1, Active transcription factor CREB antibody, cAMP responsive element binding protein 1 antibody, CREB 1 antibody, CREB-1, CREB1 antibody, MGC9284 antibody, Transactivator protein antibody
- 纯化方法
- Anti-CREB pS133 was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first sequentially pre-adsorbed against a control E.
- 过滤
- Sterile filtered
- 免疫原
-
Immunogen: CREB phospho peptide corresponding to amino acid residues 122-147 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product CREB1 Primary Antibody
-
anti-cAMP Responsive Element Binding Protein 1 (CREB1) antibody
CREB1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 5G3 unconjugated
anti-cAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibodyCREB1 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-cAMP Responsive Element Binding Protein 1 (CREB1) (pSer142) antibodyCREB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-cAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibodyCREB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-cAMP Responsive Element Binding Protein 1 (CREB1) (AA 1-341) antibodyCREB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3F1-1B2 unconjugated
anti-cAMP Responsive Element Binding Protein 1 (CREB1) (AA 1-95) antibodyCREB1 适用: 人 WB, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 5F2 unconjugated
anti-cAMP Responsive Element Binding Protein 1 (CREB1) (AA 1-341) antibodyCREB1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-cAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibodyCREB1 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB05198 unconjugated
anti-cAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibodyCREB1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Monoclonal 3C11 unconjugated Recombinant Antibody
anti-cAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibodyCREB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Immunohistochemistry Dilution: 20 μg/mL
Application Note: Anti-CREB pS133 antibody reacts with phosphorylated human CREB at pS133 and shows minimal reactivity by western blot with non-phosphorylated CREB and minimal reactivity (1 % ) by ELISA against the non-phosphorylated form of the immunizing peptide. This antibody was assayed against a variety of tissues including fibroblasts and B-cell lysates. Bands of 46 and 43 kDa corresponding to phosphorylated CREB are observed in western blots. Anti-CREB pS133 is suitable for the detection by immunoblot of phosphorylated human, mouse and rat CREB. No cross-reactivity occurs with non-phosphorylated CREB. For immunohistochemistry, formalin fixed, paraffin embedded human tissue shows moderate to strong nuclear staining in a variety of cells with minimal background staining. Although not tested this antibody is likely functional by FACS and immunoprecipitation.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:1,000 - 1:6,000
Other: User Optimized
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.064 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
-
: "Sirtuin 1 (SIRT1) deacetylase activity is not required for mitochondrial biogenesis or peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) deacetylation following ..." in: The Journal of biological chemistry, Vol. 286, Issue 35, pp. 30561-70, (2011) (PubMed).
-
: "Sirtuin 1 (SIRT1) deacetylase activity is not required for mitochondrial biogenesis or peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) deacetylation following ..." in: The Journal of biological chemistry, Vol. 286, Issue 35, pp. 30561-70, (2011) (PubMed).
-
- 抗原
- CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))
- 别名
- CREB1 (CREB1 产品)
- 背景
- Background: The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMP-dependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
- 基因ID
- 1385
- UniProt
- P16220
- 途径
- TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation
-
