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CREB1 抗体 (pSer133)

Name: CREB1 抗体 (pSer133)
SKU: ABIN723980
Availability: OutOfStock

CREB1 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC (p), FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

U251 lysates probed with CREB-1(Ser133) Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-phospho-CREB-1(Ser133) Polyclonal Antibody (ABIN723980) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

IHC

Formalin-fixed and paraffin embedded mouse hippocampus labeled with Rabbit Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated 1:500 followed by conjugation to the secondary antibody

IHC

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated 1:500 followed by conjugation to the secondary antibody

IHC

Rat RSC96 cells probed with CREB1 (Ser133) Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

FACS

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-phospho-CREB-1(Ser133) Polyclonal Antibody, Unconjugated (ABIN723980) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Mouse spleen lysate probed with Anti-CREB-1(Ser133) Polyclonal Antibody at 1:5000 90min in 37˚C.

7 images

(1 reference)

产品编号 ABIN723980

发货至: 中国

Datasheet as PDF

抗原 See all CREB1 抗体

CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))
抗原表位
63

29

20

17

16

16

10

8

8

8

8

7

7

6

5

4

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer133
适用
288

210

176

32

24

18

17

14

12

10

9

8

8

5

3

3

1

1

1

1
人, 小鼠, 大鼠, Pig
宿主
264

26

1

1
兔
Compatible Secondaries
克隆类型
239

53
多克隆
标记
148

16

13

12

11

11

11

11

11

10

9

5

5

5

5

5

4
This CREB1 antibody is un-conjugated
应用范围
224

99

97

74

55

55

47

47

20

17

16

6

5

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, Pig, 大鼠

预测反应

Dog,Cow,Sheep,Chicken

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic phosphopeptide derived from human CREB-1 around the phosphorylation site of Ser133 [RP(p-S)YR]

亚型

IgG

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anti-cAMP Responsive Element Binding Protein 1 (CREB1) (pSer142) antibody
CREB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-cAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody
CREB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-cAMP Responsive Element Binding Protein 1 (CREB1) (AA 1-341) antibody
CREB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3F1-1B2 unconjugated

anti-cAMP Responsive Element Binding Protein 1 (CREB1) (AA 1-95) antibody
CREB1 适用: 人 WB, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 5F2 unconjugated

anti-cAMP Responsive Element Binding Protein 1 (CREB1) (AA 1-341) antibody
CREB1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-cAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody
CREB1 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB05198 unconjugated

anti-cAMP Responsive Element Binding Protein 1 (CREB1) antibody
CREB1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, IP, ICC 宿主: 兔 Monoclonal unconjugated

anti-cAMP Responsive Element Binding Protein 1 (CREB1) antibody
CREB1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-cAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody
CREB1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Monoclonal 3C11 unconjugated Recombinant Antibody

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Xu, Xu, Luan, Jiang, Tian, Zhang: "Cardioprotection against experimental myocardial ischemic injury using cornin." in: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, Vol. 49, Issue 2, pp. e5039, (2016) (PubMed).
抗原

CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

别名

CREB-1 (CREB1 产品)

别名

creb antibody, CREB1 antibody, Creb1 antibody, CREB antibody, 2310001E10Rik antibody, 3526402H21Rik antibody, AV083133 antibody, Creb antibody, Creb-1 antibody, creb1 antibody, zgc:55598 antibody, cAMP responsive element binding protein 1 antibody, cAMP responsive element binding protein antibody, cAMP responsive element binding protein 1 S homeolog antibody, cAMP responsive element binding protein 1a antibody, CREB1 antibody, creb1 antibody, CREB antibody, Creb1 antibody, creb1.S antibody, creb1a antibody

背景

Synonyms: CREB, Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1
Background: Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

基因ID

1385

UniProt

P16220

途径

TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation