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beta Actin 抗体 (N-Term)

Name: beta Actin 抗体 (N-Term)
Brand: antibodies-online
SKU: ABIN968977
Availability: OutOfStock

ACTB 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 8H10D10 unconjugated

Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate. (beta Actin 抗体 (N-Term))

Flow cytometric analysis of MCF-7 cells using beta Actin mouse mAb (right) and negative control (left). (beta Actin 抗体 (N-Term))

FACS

Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta Actin mouse mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG). (beta Actin 抗体 (N-Term))

3 images

(5 references)

产品编号 ABIN968977

发货至: 中国

Datasheet as PDF

抗原 See all beta Actin (ACTB) 抗体

beta Actin (ACTB) (Actin, beta (ACTB))
抗原表位
40

16

15

6

6

4

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
适用
177

131

111

55

42

42

37

27

25

23

18

17

12

10

8

7

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1
人
宿主
113

80

5

1

1
小鼠
Compatible Secondaries
克隆类型
112

86
单克隆
标记
126

12

12

10

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This beta Actin antibody is un-conjugated
应用范围
167

73

52

46

33

30

25

21

13

13

8

6

6

6

5

2

1

1

1
Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

原理

beta-Actin Antibody

纯化方法

Ascitic fluid

免疫原

Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.

克隆位点

8H10D10

亚型

IgG2b

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anti-Actin, beta (ACTB) (AA 2-16) antibody
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anti-Actin, beta (ACTB) (AA 350-375) antibody
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anti-Actin, beta (ACTB) (AA 1-25) antibody
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anti-Actin, beta (ACTB) (AA 1-375) antibody
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anti-Actin, beta (ACTB) (N-Term) antibody
KO Validated ACTB 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB 适用: 人, 小鼠, 小鸡, 兔, Pig, Cow, Hamster, 山羊, 鱼 WB, ELISA 宿主: 小鼠 Monoclonal 2D1D10 unconjugated

anti-Actin, beta (ACTB) antibody
ACTB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), FACS, DNA Mic 宿主: 小鼠 Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB 适用: 人 WB, ELISA, IHC, IF, IP, FACS 宿主: 小鼠 Monoclonal 1D12E12B5 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
ACTB 适用: 人, 小鼠, 大鼠, 小鸡, 兔, 犬, 猴, Pig, Cow, 绵羊, Hamster, 马, 豚鼠, 山羊, Cat, Avian, 驴 WB, IF 宿主: 山羊 Polyclonal unconjugated

应用备注

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/200 - 1/1000

限制

仅限研究用
状态

Liquid

缓冲液

Ascitic fluid containing 0.03 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Zou, Ren, Liu, Fu, Chen, Li, Luo, He, Gao, Zeng, Xiong, Li, Huang, Xu, Zhang: "Inhibin B suppresses anoikis resistance and migration through the transforming growth factor-β signaling pathway in nasopharyngeal carcinoma." in: Cancer science, Vol. 109, Issue 11, pp. 3416-3427, (2018) (PubMed).

Rual, Venkatesan, Hao, Hirozane-Kishikawa, Dricot, Li, Berriz, Gibbons, Dreze, Ayivi-Guedehoussou, Klitgord, Simon, Boxem, Milstein, Rosenberg, Goldberg, Zhang, Wong, Franklin, Li, Albala, Lim et al.: "Towards a proteome-scale map of the human protein-protein interaction network. ..." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, (2005) (PubMed).

Xu, Wratten, Charych, Buyske, Firestein, Brzustowicz: "Increased expression in dorsolateral prefrontal cortex of CAPON in schizophrenia and bipolar disorder." in: PLoS medicine, Vol. 2, Issue 10, pp. e263, (2005) (PubMed).

Pederson, Aebi: "Nuclear actin extends, with no contraction in sight." in: Molecular biology of the cell, Vol. 16, Issue 11, pp. 5055-60, (2005) (PubMed).

Bruneel, Labas, Mailloux, Sharma, Royer, Vinh, Pernet, Vaubourdolle, Baudin: "Proteomics of human umbilical vein endothelial cells applied to etoposide-induced apoptosis." in: Proteomics, Vol. 5, Issue 15, pp. 3876-84, (2005) (PubMed).
抗原

beta Actin (ACTB) (Actin, beta (ACTB))

别名

beta-Actin (ACTB 产品)

背景

Description: Beta-actin (PS1TP5-binding protein 1), also known as ACTB, PS1TP5BP1. Entrez Protein NP_001092. It is one of six different actin proteins. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton.Actins are highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Actin is ubiquitously expressed in all eukaryotic cells. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Aliases: PS1TP5BP1, ACTB

分子量

42kDa

基因ID

60

HGNC

60

UniProt

P60709

途径

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction