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LIM Domain Kinase 1 抗体 (AA 232-333)

Name: LIM Domain Kinase 1 抗体 (AA 232-333)
SKU: ABIN968654
Availability: OutOfStock

This anti-LIM Domain Kinase 1 antibody is a 小鼠单克隆 antibody detecting LIM Domain Kinase 1 in WB 和 IF. Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 3+ publications.

Western blot analysis of LIMK1 on rat cerebellum lysate. (LIM Domain Kinase 1 抗体 (AA 232-333))

Immunofluorescent staining of NIH-3T3 cells. (LIM Domain Kinase 1 抗体 (AA 232-333))

2 images

(3 references)

产品编号 ABIN968654

发货至: 中国

Datasheet as PDF

Quick Overview for LIM Domain Kinase 1 抗体 (AA 232-333) (ABIN968654)

抗原

See all LIM Domain Kinase 1 (LIMK1) 抗体

LIM Domain Kinase 1 (LIMK1)

适用

人, 小鼠, 大鼠

宿主

小鼠

克隆类型

单克隆

标记

This LIM Domain Kinase 1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF)

克隆位点

42-LIMK1

Discover our top product LIMK1 Primary Antibody

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AA 232-333

交叉反应

大鼠, 小鼠

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human LIMK1 aa. 232-333

亚型

IgG1
anti-LIM Domain Kinase 1 (LIMK1) (pThr508) antibody
LIMK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-LIM Domain Kinase 1 (LIMK1) antibody
LIMK1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 3H6 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) antibody
LIMK1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 6B4 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 451-550) antibody
LIMK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 548-647) antibody
LIMK1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1B2 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 548-647) antibody
LIMK1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A8 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 548-647) antibody
LIMK1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2E9 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (pThr508) antibody
LIMK1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (pThr508) antibody
LIMK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 1-647) antibody
LIMK1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

说明

Related Products: ABIN968546, ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20°C.
Ohashi, Nagata, Maekawa, Ishizaki, Narumiya, Mizuno: "Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop." in: The Journal of biological chemistry, Vol. 275, Issue 5, pp. 3577-82, (2000) (PubMed).

Edwards, Gill: "Structural features of LIM kinase that control effects on the actin cytoskeleton." in: The Journal of biological chemistry, Vol. 274, Issue 16, pp. 11352-61, (1999) (PubMed).

Frangiskakis, Ewart, Morris, Mervis, Bertrand, Robinson, Klein, Ensing, Everett, Green, Pröschel, Gutowski, Noble, Atkinson, Odelberg, Keating: "LIM-kinase1 hemizygosity implicated in impaired visuospatial constructive cognition." in: Cell, Vol. 86, Issue 1, pp. 59-69, (1996) (PubMed).
抗原

LIM Domain Kinase 1 (LIMK1)

别名

LIMK1

背景

Two LIM motif-containing protein kinases (LIMK) have been identified, LIMK1 and LIMK2. These kinases contain two N-terminal LIM domains, a central PDZ domain, and a C-terminal Ser/Thr kinase domain. LIMK1 is highly expressed in brain, heart, and skeletal muscle, while LIMK2 exhibits the highest expression in placenta, liver, lung, kidney, and pancreas. LIMK1 is localized to the actin cytoskeleton and phosphorylates the actin binding/depolymerizing factor, cofilin. During Rho-induced neurite retraction, activation of ROCK leads to LIMK1 activation via phosphorylation at Thr508. In COS-7 cells, disruption of the second LIM domain or the PDZ domain increases LIMK1-induced aggregation of the actin cytoskeleton. In addition, a 32 kDa splice variant that contains only the N-terminus (dLIMK1) suppresses LIMK1 activity by interaction with the C- terminal kinase domain. In humans, deletion of LIMK1 has been implicated in Williams syndrome, a disorder that produces a distinct cognitive profile and vascular disease. Thus, LIMK1, and its splice variant dLIMK1, are thought to have important roles in the regulation of the actin cytoskeleton in a wide varitey of tissues.

分子量

72 kDa

途径

Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events