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LIM Domain Kinase 1 抗体 (pThr508)

LIMK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of extracts from Hela cells,Hela+H2o2 cells and p-peptide Rat brain tissue, using Phospho-LIMK1 (Thr508) Antibody(af3345) at 1/1000 dilution. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/200 staining human gastric tissue sections by ICC/IF. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/200 staining human heart tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining rat gastric tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining rat liver tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining rat tumor tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining mouse splenic tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/200 staining Mouse spleen tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/200 staining human lymphoma tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining rat lung tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining mouse testicular tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/200 staining human liver tissue cells by ICC/IF. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/200 staining human renal clear cell carcinoma tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/200 staining human colon cancer tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/200 staining human seminoma tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining rat appendiceal tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining mouse lung tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining mouse pancreatic tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/200 staining Human lung cancer tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/200 staining Human lung cancer tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining rat kidney tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining rat uterine tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/200 staining human brain tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining mouse gastric tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/200 staining human lung cancer tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 staining NIH-3T3 by IF/ICC. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/200 staining human gastric tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining rat thymus tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/200 staining human vascular cancer tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining rat splenic tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/200 staining human esophageal carcinoma tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining rat brain tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/200 staining human liver cancer tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/200 staining human duodenum tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining rat testicular tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/200 staining Mouse spleen tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining rat heart tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining rat ovarian tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining rat intestinal tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining mouse kidney tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 at 1/100 staining mouse brain tissue sections by IHC-P. (LIM Domain Kinase 1 抗体 (pThr508))

Western blot analysis of LIMK1 phosphorylation expression in COLO205 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (LIM Domain Kinase 1 抗体 (pThr508))

ABIN6267554 staining NIH-3T3 by IF/ICC. (LIM Domain Kinase 1 抗体 (pThr508))

43 images

产品编号 ABIN6255701

发货至: 中国

Datasheet as PDF

抗原 See all LIM Domain Kinase 1 (LIMK1) 抗体

LIM Domain Kinase 1 (LIMK1)
抗原表位
23

15

15

8

7

7

6

5

4

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr508
适用
101

80

75

2

2

1

1

1

1

1
人, 小鼠, 大鼠
宿主
101

15

3
兔
Compatible Secondaries
克隆类型
105

14
多克隆
标记
52

10

8

5

5

5

5

5

5

5

2

2

2

2

2

2

2
This LIM Domain Kinase 1 antibody is un-conjugated
应用范围
83

50

26

26

23

19

9

8

7

6

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

特异性

Phospho-LIMK1 (Thr508) Antibody detects endogenous levels of LIMK1 only when phosphorylated at Threonine 508.

预测反应

Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human LIMK1 around the phosphorylation site of Thr508.

亚型

IgG
anti-LIM Domain Kinase 1 (LIMK1) antibody
LIMK1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 3H6 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) antibody
LIMK1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 6B4 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 451-550) antibody
LIMK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 548-647) antibody
LIMK1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A8 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 548-647) antibody
LIMK1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1B2 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 548-647) antibody
LIMK1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2E9 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (pThr508) antibody
LIMK1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (pThr508) antibody
LIMK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 1-647) antibody
LIMK1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-LIM Domain Kinase 1 (LIMK1) antibody
LIMK1 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
抗原

LIM Domain Kinase 1 (LIMK1)

别名

LIMK1 (LIMK1 产品)

别名

limk1 antibody, xlimk1 antibody, GB16654 antibody, LIMK1 antibody, LIMK antibody, LIMK-1 antibody, LIM domain kinase 1 antibody, LIM domain kinase 1a antibody, LIM domain kinase 1 L homeolog antibody, LIM-domain containing, protein kinase antibody, LIMK1 antibody, limk1a antibody, limk1 antibody, CpipJ_CPIJ000717 antibody, LOC413152 antibody, Limk1 antibody, limk1.L antibody

背景

Description: Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).
Gene: LIMK1

分子量

70kDa

基因ID

3984

UniProt

P53667

途径

Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events

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