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LIM Domain Kinase 1 抗体 (AA 451-550)

Name: LIM Domain Kinase 1 抗体 (AA 451-550)
SKU: ABIN680518
Availability: OutOfStock

LIMK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-LIMK1 Polyclonal Antibody, Unconjugated (ABIN680518) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (LIM Domain Kinase 1 抗体 (AA 451-550))

IHC

Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with LIMK1 Polyclonal Antibody (bs-2775R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining. (LIM Domain Kinase 1 抗体 (AA 451-550))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse brain, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with LIMK1 Polyclonal Antibody (bs-2775R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining. (LIM Domain Kinase 1 抗体 (AA 451-550))

IHC (p)

3 images

(2 references)

产品编号 ABIN680518

发货至: 中国

Datasheet as PDF

抗原 See all LIM Domain Kinase 1 (LIMK1) 抗体

LIM Domain Kinase 1 (LIMK1)
抗原表位
24

15

14

8

7

7

6

5

4

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 451-550
适用
101

80

75

2

2

1

1

1

1

1
人, 小鼠, 大鼠
宿主
101

15

3
兔
Compatible Secondaries
克隆类型
105

14
多克隆
标记
52

10

8

5

5

5

5

5

5

5

2

2

2

2

2

2

2
This LIM Domain Kinase 1 antibody is un-conjugated
应用范围
83

50

25

25

24

18

10

8

7

5

2

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, 大鼠

预测反应

Cow,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human LIMK1

亚型

IgG

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anti-LIM Domain Kinase 1 (LIMK1) antibody
LIMK1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 3H6 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) antibody
LIMK1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 6B4 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 548-647) antibody
LIMK1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A8 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 548-647) antibody
LIMK1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1B2 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 548-647) antibody
LIMK1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2E9 unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (pThr508) antibody
LIMK1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (pThr508) antibody
LIMK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-LIM Domain Kinase 1 (LIMK1) (AA 1-647) antibody
LIMK1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-LIM Domain Kinase 1 (LIMK1) antibody
LIMK1 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Zhou, Liu, Luo, Zhou, Xia, Chen, Zhou, Zou, Cao, Cao: "MiR-20a inhibits cutaneous squamous cell carcinoma metastasis and proliferation by directly targeting LIMK1." in: Cancer biology & therapy, Vol. 15, Issue 10, pp. 1340-9, (2015) (PubMed).
抗原

LIM Domain Kinase 1 (LIMK1)

别名

LIMK1 (LIMK1 产品)

别名

limk1 antibody, xlimk1 antibody, GB16654 antibody, LIMK1 antibody, LIMK antibody, LIMK-1 antibody, LIM domain kinase 1 antibody, LIM domain kinase 1a antibody, LIM domain kinase 1 L homeolog antibody, LIM-domain containing, protein kinase antibody, LIMK1 antibody, limk1a antibody, limk1 antibody, CpipJ_CPIJ000717 antibody, LOC413152 antibody, Limk1 antibody, limk1.L antibody

背景

Synonyms: LIMK, LIMK-1, LIM domain kinase 1, LIMK1
Background: Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).

基因ID

3984

UniProt

P53667

途径

Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events