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CYBB 抗体 (AA 450-556)

Name: CYBB 抗体 (AA 450-556)
SKU: ABIN968524
Availability: OutOfStock

This anti-CYBB antibody is a 小鼠单克隆 antibody detecting CYBB in WB 和 IF. Suitable for 小鼠和大鼠. This Primary Antibody has been cited in 4+ publications.

Western blot analysis of gp91[phox] on mouse macrophage lysate. (CYBB 抗体 (AA 450-556))

Immunofluorescent staining of mouse macrophage cells with gp91[phox] antibody. (CYBB 抗体 (AA 450-556))

Image no. 3 for anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 450-556) antibody (ABIN968524) (CYBB 抗体 (AA 450-556))

3 images

(4 references)

产品编号 ABIN968524

发货至: 中国

Quick Overview for CYBB 抗体 (AA 450-556) (ABIN968524)

抗原

See all CYBB 抗体

CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

适用

小鼠, 大鼠

宿主

小鼠

克隆类型

单克隆

标记

This CYBB antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF)

克隆位点

53-gp91[phox]

Discover our top product CYBB Primary Antibody

抗原表位
16

9

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2

1

1

1

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1

1

1

1

1

1

1

1
AA 450-556

交叉反应

大鼠

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Mouse gp91[phox] aa. 450-556

亚型

IgG1
anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 501-570) antibody
CYBB 适用: 人, 小鼠, 大鼠, 兔, 小鸡 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 283-570) antibody
CYBB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 283-570) antibody
CYBB 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (C-Term) antibody
CYBB 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) antibody
CYBB 适用: 人 WB, IF, IHC (p), ICC 宿主: 小鼠 Monoclonal 54-1 unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 442-459), (Middle Region) antibody
CYBB 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 283-570) antibody
CYBB 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (Internal Region) antibody
Verified CYBB 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 100-200) antibody
CYBB 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Cytochrome B-245, beta Polypeptide (CYBB) (AA 416-500) antibody
CYBB 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated

说明

Related Products: ABIN968555, ABIN967389, ABIN967918, ABIN968245

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20°C.
Jacobsen, Skalnik: "YY1 binds five cis-elements and trans-activates the myeloid cell-restricted gp91(phox) promoter." in: The Journal of biological chemistry, Vol. 274, Issue 42, pp. 29984-93, (1999) (PubMed).

Suzuki, Kumatori, Haagen, Fujii, Sadat, Jun, Tsuji, Roos, Nakamura: "PU.1 as an essential activator for the expression of gp91(phox) gene in human peripheral neutrophils, monocytes, and B lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 11, pp. 6085-90, (1998) (PubMed).

Yu, Quinn, Cross, Dinauer: "Gp91(phox) is the heme binding subunit of the superoxide-generating NADPH oxidase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 14, pp. 7993-8, (1998) (PubMed).

Zhen, Yu, Dinauer: "Probing the role of the carboxyl terminus of the gp91phox subunit of neutrophil flavocytochrome b558 using site-directed mutagenesis." in: The Journal of biological chemistry, Vol. 273, Issue 11, pp. 6575-81, (1998) (PubMed).
抗原

CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

别名

gp91 phox

背景

Although dormant in resting granulocytes, macrophages, and B lymphocytes, the neutrophil respiratory burst oxidase (NADPH-oxidase) generates superoxide and secondary oxygen-derived toxic products in response to bacteria or a variety of soluble stimuli. It is an integral membrane cytochrome, b558, which consists of two subunits, gp91[phox] and p21[phox]. Upon stimulation, cytochrome b558 forms a complex with cytosolic proteins, p67[phox], p47[phox], p40[phox], and rac2 and produces superoxide anions in a NADPH-dependent manner. In chronic granulomatous disease (CGD), severe recurrent bacterial and fungal infections result from defective NADPH-oxidase activity. The majority of CGD cases are caused by a defective gp91[phox] gene. gp91[phox], implicated as a docking site for p47[phox], is membrane glycoprotein with multiple N-terminal hydrophobic domains and a hydrophilic C-terminus. The expression of gp91[phox] is restricted to terminally differentiated phagocytes and B lymphocytes. This cell type- and developmental stage-specific expression may be controlled by transcriptional activators, such as PU.1 and YY1, and transcriptional repressors, such as CCAAT displacement protein (CDP). Endogenous mouse gp91phox shows 58 kDa band, instead of 91 kDa observed in human. Both mouse and human deglycosylated gp91 are 54 kDa. This difference may be due to less glycosylation sites in the mouse sequence.

分子量

58 kDa