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GIT1 抗体 (AA 664-770)

Name: GIT1 抗体 (AA 664-770)
SKU: ABIN968516
Availability: OutOfStock

This anti-GIT1 antibody is a 小鼠单克隆 antibody detecting GIT1 in WB 和 IF. Suitable for 人, 大鼠和小鼠. This Primary Antibody has been cited in 2+ publications.

Western blot analysis of GIT1 on a rat cerebrum lysate. (GIT1 抗体 (AA 664-770))

Immunofluorescence staining of A431cells (Human epithelial carcinoma, ATCC CRL-1555) with the mouse anti-GIT1 antibody. (GIT1 抗体 (AA 664-770))

2 images

(2 references)

产品编号 ABIN968516

发货至: 中国

Datasheet as PDF

Quick Overview for GIT1 抗体 (AA 664-770) (ABIN968516)

抗原

See all GIT1 抗体

GIT1

适用

人, 大鼠, 小鼠

宿主

小鼠

克隆类型

单克隆

标记

This GIT1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF)

克隆位点

13-GIT1

Discover our top product GIT1 Primary Antibody

抗原表位
17

15

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9

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7

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 664-770

交叉反应

小鼠, 人

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Rat GIT1 aa. 664-770

亚型

IgG2a
anti-GIT1 (GIT1) (AA 460-640) antibody
KO Validated GIT1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-GIT1 (GIT1) (AA 470-642) antibody
GIT1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-GIT1 (GIT1) (AA 533-561), (C-Term), (Tyr554) antibody
GIT1 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB15351 unconjugated

anti-GIT1 (GIT1) (Internal Region) antibody
GIT1 适用: 人, 大鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-GIT1 (GIT1) (Internal Region) antibody
GIT1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-GIT1 (GIT1) (AA 375-770) antibody
GIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 unconjugated

anti-GIT1 (GIT1) (AA 375-770) antibody (HRP)
GIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 HRP

anti-GIT1 (GIT1) (AA 375-770) antibody (FITC)
GIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 FITC

anti-GIT1 (GIT1) (AA 375-770) antibody (Biotin)
GIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 Biotin

anti-GIT1 (GIT1) (AA 375-770) antibody (PE)
GIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 PE

说明

Related Products: ABIN968545, ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20°C.
Zhao, Manser, Loo, Lim: "Coupling of PAK-interacting exchange factor PIX to GIT1 promotes focal complex disassembly." in: Molecular and cellular biology, Vol. 20, Issue 17, pp. 6354-63, (2000) (PubMed).

Premont, Claing, Vitale, Freeman, Pitcher, Patton, Moss, Vaughan, Lefkowitz: "beta2-Adrenergic receptor regulation by GIT1, a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 24, pp. 14082-7, (1998) (PubMed).
抗原

GIT1

别名

GIT1

背景

Activation of adrenergic receptors (AR), such as beta2AR leads to activation of heterotrimeric G proteins and recruitment of G protein-coupled receptor kinases (GRKs). Phosphorylation of the activated receptor by GRKs promotes the binding of arrestin protein which prevents further G protein activation. GRK interactor 1 (GIT1) was identified in a yeast two-hybrid screen for proteins that bind GRK2. GIT1 has an N-terminal Zn2+ finger-like motif involved in GTPase activating activity and multipe ankyrin repeat units. In vitro, GIT1 is a GTPase-activating protein (GAP) for the ADP ribosylation factor (ARF) family of small GTP-binding proteins. In rat, GIT1 is widely expressed with the highest levels in the testis and lowest levels in the liver and spleen. Overexpression of GIT1 in HEK293 cells causes reduced beta2AR signaling and increased receptor phosphorylation as a result of reduced receptor internalization and resensitization. In addition, GIT1 interacts directly with paxillin and the PIX exchange factors, and indirectly with PAKs, Rac1, and Nck. GIT proteins are also now known to be PIP3-stimulated GAPs for ARF6. Thus, GIT1 activation of ARF proteins may involve cell signaling pathways that are important for endocytosis, cell adhesion, and cytoskeletal dynamics.
Synonyms: G protein-coupled receptor kinase interactor 1

分子量

95 kDa