我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

Calnexin 抗体 (AA 116-301)

Name: Calnexin 抗体 (AA 116-301)
SKU: ABIN968018
Availability: OutOfStock

CANX 适用: 人, 小鼠, 大鼠, 犬 WB, IHC, IP, BI 宿主: 小鼠 Monoclonal 37-Calnexin unconjugated

Western blot analysis of Calnexin on a HeLa lysate. (Calnexin 抗体 (AA 116-301))

Immunofluorescent staining of A549 (ATCC CCL-185) cells. (Calnexin 抗体 (AA 116-301))

Image no. 3 for anti-Calnexin (CANX) (AA 116-301) antibody (ABIN968018) (Calnexin 抗体 (AA 116-301))

3 images

(4 references)

产品编号 ABIN968018

发货至: 中国

Datasheet as PDF

抗原 See all Calnexin (CANX) 抗体

Calnexin (CANX)
抗原表位
49

17

14

6

5

5

5

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 116-301
适用
117

73

72

48

34

31

31

29

29

26

25

25

22

21

20

6

3

3

2

2

2

1

1

1

1

1

1

1

1
人, 小鼠, 大鼠, 犬
宿主
117

23

7
小鼠
Compatible Secondaries
克隆类型
114

34
单克隆
标记
71

8

7

5

5

4

4

4

3

3

3

3

3

3

3

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Calnexin antibody is un-conjugated
应用范围
123

79

57

56

39

28

27

26

21

14

13

9

8

4

3

3

2

2

2

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), BioImaging (BI)

交叉反应

小鼠, 大鼠, 犬

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
3. Triton is a trademark of the Dow Chemical Company.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. Please refer to us for technical protocols.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human Calnexin aa. 116-301

克隆位点

37-Calnexin

亚型

IgG1

Top Product

Discover our top product CANX Primary Antibody
anti-Calnexin (CANX) (C-Term) antibody
CANX 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 豚鼠, Pig, 猴, 绵羊, Hamster, 小鸡, Avian, 马, 山羊, Cat, 驴 WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated

anti-Calnexin (CANX) (C-Term) antibody
CANX 适用: 犬 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Calnexin (CANX) (Internal Region) antibody
CANX 适用: 人, 小鼠, 大鼠 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Calnexin (CANX) (AA 263-482) antibody
CANX 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Calnexin (CANX) (AA 1-300), (N-Term) antibody
CANX 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal CANX-1541 unconjugated

anti-Calnexin (CANX) (AA 504-592) antibody
CANX 适用: 人 WB, IF, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 1D4 unconjugated

anti-Calnexin (CANX) (AA 239-461) antibody
CANX 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Calnexin (CANX) (AA 239-461) antibody
CANX 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated

anti-Calnexin (CANX) (AA 1-300), (N-Term) antibody
CANX 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal CANX-1543 unconjugated

anti-Calnexin (CANX) (C-Term) antibody
CANX 适用: 犬 WB, IHC, IF, IP, FACS, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.

说明

Related Products: ABIN967389, ABIN968535

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20°C.
Nagahama, Suzuki, Hamada, Hatsuzawa, Tani, Yamamoto, Tagaya: "SVIP is a novel VCP/p97-interacting protein whose expression causes cell vacuolation." in: Molecular biology of the cell, Vol. 14, Issue 1, pp. 262-73, (2003) (PubMed).

Gkantiragas, Brügger, Stüven, Kaloyanova, Li, Löhr, Lottspeich, Wieland, Helms: "Sphingomyelin-enriched microdomains at the Golgi complex." in: Molecular biology of the cell, Vol. 12, Issue 6, pp. 1819-33, (2001) (PubMed).

Lee, Kim, Choi, Mehl, Jung, Gil, Rowe, Park: "CD99 expression is positively regulated by Sp1 and is negatively regulated by Epstein-Barr virus latent membrane protein 1 through nuclear factor-kappaB." in: Blood, Vol. 97, Issue 11, pp. 3596-604, (2001) (PubMed).

Tjoelker, Seyfried, Eddy, Byers, Shows, Calderon, Schreiber, Gray: "Human, mouse, and rat calnexin cDNA cloning: identification of potential calcium binding motifs and gene localization to human chromosome 5." in: Biochemistry, Vol. 33, Issue 11, pp. 3229-36, (1994) (PubMed).
抗原

Calnexin (CANX)

别名

Calnexin (CANX 产品)

别名

CNX antibody, IP90 antibody, P90 antibody, 1110069N15Rik antibody, AI988026 antibody, Cnx antibody, D11Ertd153e antibody, canx antibody, clgn antibody, clnx antibody, cnx antibody, PP90 antibody, zgc:63524 antibody, wu:fe06b12 antibody, CANX antibody, calnexin antibody, CalNeXin antibody, calnexin L homeolog antibody, CANX antibody, Canx antibody, cnx-1 antibody, canx.L antibody, canx antibody, LOC100283947 antibody

背景

Calnexin is a 90 kDa integral membrane protein located primarily in the ER. It contains a long N-terminal calcium-binding domain that extends into the lumen of the ER and a short, acidic cytosolic domain. Calnexin associates with several cell surface proteins as they pass through the ER. Since it also forms complexes with other resident ER proteins involved in the Ca2+-dependent retention of proteins, calnexin may act as a chaperone. The amino acid sequence of calnexin is highly conserved among species and is similar in sequence to calreticulin, another Ca2+-binding protein found in the ER. Neither calnexin nor calreticulin contain the calcium-binding motif, known as the E-F hand, found in the calmodulin family of proteins.

分子量

90 kDa

途径

MAPK Pathway, Thyroid Hormone Synthesis