Calnexin 抗体 (N-Term)
Our Local Distributor
北京 101111
Quick Overview for Calnexin 抗体 (N-Term) (ABIN6940953)
抗原
See all Calnexin (CANX) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 1-300, N-Term
-
原理
- Calnexin (Endoplasmic Reticulum Marker) Antibody
-
特异性
- It recognizes a protein of 90 kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.
-
纯化方法
- 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.
-
免疫原
- Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)
-
亚型
- IgG1, kappa
-
-
anti-Calnexin (CANX) (C-Term) antibody
CANX 适用: 犬 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calnexin (CANX) (Internal Region) antibodyCANX 适用: 人, 小鼠, 大鼠 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Calnexin (CANX) (C-Term) antibodyCANX 适用: 人, 小鼠, 大鼠, 犬, Cow, 猴, 兔, 豚鼠, Pig, Hamster, 绵羊, 小鸡, Avian, 马, 山羊, Cat, 驴 WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-Calnexin (CANX) (AA 263-482) antibodyCANX 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Calnexin (CANX) (AA 504-592) antibodyCANX 适用: 人 WB, IF, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Calnexin (CANX) (AA 1-300), (N-Term) antibodyCANX 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal CANX-1541 unconjugated
anti-Calnexin (CANX) (C-Term) antibody (Atto 390)CANX 适用: 犬 WB, IHC, IF, ICC, IP, FACS 宿主: 兔 Polyclonal Atto 390
anti-Calnexin (CANX) (C-Term) antibody (Atto 488)CANX 适用: 犬 WB, IHC, IF, ICC, IP, FACS 宿主: 兔 Polyclonal Atto 488
anti-Calnexin (CANX) (C-Term) antibody (Atto 594)CANX 适用: 犬 WB, IHC, IF, ICC, IP, FACS 宿主: 兔 Polyclonal Atto 594
anti-Calnexin (CANX) (C-Term) antibody (APC)CANX 适用: 犬 WB, IHC, IF, ICC, IP, FACS 宿主: 兔 Polyclonal APC
-
-
应用备注
-
Positive Control: HeLa, MCF-7 or U2OS cells. Kidney or small intestine.
Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
-
限制
- 仅限研究用
-
-
-
浓度
- 200 μg/mL
-
缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
-
有效期
- 24 months
-
-
- Calnexin (CANX)
-
别名
- CANX
-
背景
-
Calnexin, CANX, CNX, IP90, Major histocompatibility complex class I antigen-binding protein p88, P90
HeLa, MCF-7 or U2OS cells. Kidney or small intestine.
-
分子量
- ~90kDa
-
基因ID
- 821, 567968
-
UniProt
- P27824
-
途径
- MAPK Pathway, Thyroid Hormone Synthesis
抗原
-
