FAK 抗体 (PTK2 Protein tyrosine Kinase 2) (AA 354-533)

Details for Product anti-PTK2 Antibody No. ABIN967737, 供应商: Log in to see
抗原
  • FADK
  • FAK
  • FAK1
  • FRNK
  • PPP1R71
  • p125FAK
  • pp125FAK
  • PTK2
  • Fadk
  • mKIAA4203
  • fak
  • fak1a
  • ptk2
  • ptk2a
  • wu:fa18c04
  • zgc:66114
  • CG10023
  • CT28129
  • DFAK
  • DFak
  • DFak56
  • Dfak
  • Dfak56
  • DmFAK
  • Dmel\\CG10023
  • Fak56
  • Fak56D
  • fak56
  • protein tyrosine kinase 2
  • protein tyrosine kinase 2 L homeolog
  • PTK2 protein tyrosine kinase 2
  • protein tyrosine kinase 2ab
  • Focal adhesion kinase
  • PTK2
  • ptk2.L
  • Ptk2
  • ptk2ab
  • Fak
抗原表位
AA 354-533
80
67
63
51
50
35
28
22
20
16
16
15
15
15
11
10
9
8
8
7
7
6
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
适用
小鸡, 犬, 人, 小鼠, 大鼠
714
465
430
50
26
21
17
11
11
11
10
8
6
4
4
4
3
3
3
1
1
宿主
小鼠
625
114
3
2
1
1
克隆类型 (克隆位点)
单克隆 ()
标记
This FAK antibody is un-conjugated
18
17
16
12
12
11
11
11
11
11
11
11
10
10
4
2
2
2
应用范围
BioImaging (BI), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
519
194
191
142
132
116
64
34
21
9
9
7
5
3
2
选项
Supplier
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Supplier Product No.
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免疫原 Chicken FAK aa. 354-533
克隆位点 77-FAK
亚型 IgG1
交叉反应 人, 小鼠, 大鼠, 犬
产品特性 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company.
纯化方法 The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
别名 FAK (PTK2 Antibody 摘要)
背景 Focal Adhesion Kinase (FAK) is a cytoplasmic tyrosine kinase that colocalizes with integrins in focal adhesions. This cellular localization is directed by a 125 amino acid sequence at the C-terminus called the Focal Adhesion Targeting sequence (FAT). The binding of extracellular matrix ligands to integrins triggers autophosphorylation and activation of FAK. This creates binding sites for SH2 domains of intracellular signaling molecules such as src, PI3 kinase, and Grb2. FAK's ability to bind numerous structural and signaling proteins via a variety of interactions has led to substantial speculation about its function. Although FAK's precise role has not been elucidated, proposed possibilities include regulating cell motility, cell growth, cytoskeletal organization, and adhesion-dependent cell survival.
Synonyms: Focal Adhesion Kinase
分子量 116-125 kDa
途径 Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
应用备注 Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol,or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.
说明

Related Products: ABIN967389, ABIN968533

限制 仅限研究用
状态 Liquid
浓度 250 μg/mL
缓冲液 Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Store undiluted at -20°C.
厂商提供的图像
Western Blotting (WB) image for anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 354-533) antibody (ABIN967737) Western blot analysis of FAK on a A431 cell lysate (Human epithelial carcinoma, ATCC ...
Immunofluorescence (IF) image for anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 354-533) antibody (ABIN967737) Immunofluorescent staining of U2OS (ATCC HTB-96) cells. Cells were seeded in a 96-wel...
 image for anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 354-533) antibody (ABIN967737) anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 354-533) antibody (Image 3)
有引用在: Yi, Zhou, Huber, Qu, Wang, Gerdes, Li: "Nuclear compartmentalization of FAK and FRNK in cardiac myocytes." in: American journal of physiology. Heart and circulatory physiology, Vol. 290, Issue 6, pp. H2509-15, 2006 (PubMed).

Jones, Stewart: "Nuclear import of N-terminal FAK by activation of the FcepsilonRI receptor in RBL-2H3 cells." in: Biochemical and biophysical research communications, Vol. 314, Issue 1, pp. 39-45, 2004 (PubMed).

Zeng, Si, Yu, Le, Ng, Teng, Ryan, Wang, Ponniah, Pallen: "PTP alpha regulates integrin-stimulated FAK autophosphorylation and cytoskeletal rearrangement in cell spreading and migration." in: The Journal of cell biology, Vol. 160, Issue 1, pp. 137-46, 2003 (PubMed).

Kim, Feldman: "Insulin-like growth factor I prevents mannitol-induced degradation of focal adhesion kinase and Akt." in: The Journal of biological chemistry, Vol. 277, Issue 30, pp. 27393-400, 2002 (PubMed).

Kovacic-Milivojević, Roediger, Almeida, Damsky, Gardner, Ilić: "Focal adhesion kinase and p130Cas mediate both sarcomeric organization and activation of genes associated with cardiac myocyte hypertrophy." in: Molecular biology of the cell, Vol. 12, Issue 8, pp. 2290-307, 2001 (PubMed).

Huan, van Adelsberg: "Polycystin-1, the PKD1 gene product, is in a complex containing E-cadherin and the catenins." in: The Journal of clinical investigation, Vol. 104, Issue 10, pp. 1459-68, 1999 (PubMed).

Clancy, Rediske, Tang, Nijher, Frenkel, Philips, Abramson: "Outside-in signaling in the chondrocyte. Nitric oxide disrupts fibronectin-induced assembly of a subplasmalemmal actin/rho A/focal adhesion kinase signaling complex." in: The Journal of clinical investigation, Vol. 100, Issue 7, pp. 1789-96, 1997 (PubMed).

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