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FAK 抗体 (AA 396-423)

Name: FAK 抗体 (AA 396-423)
SKU: ABIN1882121
Availability: OutOfStock

PTK2 适用: 人 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB20775 unconjugated

Western blot analysis of PTK2 Antibody (Center) (ABIN1882121 and ABIN2842373) in T47D cell line lysates (35 μg/lane).

Flow cytometric analysis of K562 cells using PTK2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

FACS

Confocal immunofluorescent analysis of PTK2 Antibody (Center) (ABIN1882121 and ABIN2842373) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

Western blot analysis of PTK2 (arrow) using rabbit polyclonal PTK2 Antibody (Center) (ABIN1882121 and ABIN2842373).

Formalin-fixed and paraffin-embedded human brain tissue reacted with PTK2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

IHC (p)

5 images

(2 references)

产品编号 ABIN1882121

发货至: 中国

Datasheet as PDF

抗原 See all FAK (PTK2) 抗体

FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))
抗原表位
37

29

27

25

23

23

18

18

16

16

13

11

8

7

6

6

5

5

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
AA 396-423
适用
324

285

220

22

21

18

17

12

9

7

6

5

3

3

3

2

1

1

1

1
人
宿主
336

18

3

2

1

1
兔
Compatible Secondaries
克隆类型
327

34
多克隆
标记
177

19

16

13

13

13

13

13

13

13

10

9

9

9

9

9

2

1
This FAK antibody is un-conjugated
应用范围
297

126

122

121

107

74

68

50

31

30

25

5

2

2
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

预测反应

C, M, Rat

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This PTK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-423 amino acids from the Central region of human PTK2.

克隆位点

RB20775

亚型

Ig Fraction

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anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 700-800) antibody
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PTK2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 355-490) antibody
PTK2 适用: 人 WB, ELISA, IP, PLA 宿主: 小鼠 Monoclonal 2C3 unconjugated

anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (pTyr576) antibody
PTK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-PTK2 Protein tyrosine Kinase 2 (PTK2) antibody
PTK2 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (AA 1-680) antibody
PTK2 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-PTK2 Protein tyrosine Kinase 2 (PTK2) (pTyr861) antibody
PTK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-PTK2 Protein tyrosine Kinase 2 (PTK2) antibody
PTK2 适用: 人 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 10H7E9 unconjugated

anti-PTK2 Protein tyrosine Kinase 2 (PTK2) antibody
PTK2 适用: 人, 小鼠 WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 10H7 unconjugated

应用备注

IF: 1:10~50. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

有效期

6 months
Stowe, Turlings, Loughrin, Lewis, Tumlinson: "The chemistry of eavesdropping, alarm, and deceit." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 1, pp. 23-8, (1995) (PubMed).

Calalb, Polte, Hanks: "Tyrosine phosphorylation of focal adhesion kinase at sites in the catalytic domain regulates kinase activity: a role for Src family kinases." in: Molecular and cellular biology, Vol. 15, Issue 2, pp. 954-63, (1995) (PubMed).
抗原

FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

别名

PTK2 (PTK2 产品)

别名

FADK antibody, FAK antibody, FAK1 antibody, FRNK antibody, PPP1R71 antibody, p125FAK antibody, pp125FAK antibody, PTK2 antibody, Fadk antibody, mKIAA4203 antibody, fak antibody, fak1a antibody, ptk2 antibody, ptk2a antibody, wu:fa18c04 antibody, zgc:66114 antibody, CG10023 antibody, CT28129 antibody, DFAK antibody, DFak antibody, DFak56 antibody, Dfak antibody, Dfak56 antibody, DmFAK antibody, Dmel\\CG10023 antibody, Fak56 antibody, Fak56D antibody, fak56 antibody, protein tyrosine kinase 2 antibody, protein tyrosine kinase 2 L homeolog antibody, PTK2 protein tyrosine kinase 2 antibody, protein tyrosine kinase 2ab antibody, Focal adhesion kinase antibody, PTK2 antibody, ptk2.L antibody, Ptk2 antibody, ptk2ab antibody, Fak antibody

背景

PTK2 is a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies.

分子量

119233

NCBI登录号

NP_001186578, NP_722560

UniProt

Q05397

途径

Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling