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ARAF 抗体 (AA 183-199)

This anti-ARAF antibody is a 小鼠 单克隆 antibody detecting ARAF in WB, IHC, IF 和 IP. Suitable for 人, 小鼠, 大鼠, 犬 和 小鸡. This Primary Antibody has been cited in 4+ publications.
产品编号 ABIN967727
发货至: 中国

Quick Overview for ARAF 抗体 (AA 183-199) (ABIN967727)

抗原

See all ARAF 抗体
ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

适用

  • 109
  • 40
  • 21
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
人, 小鼠, 大鼠, 犬, 小鸡

宿主

  • 104
  • 5
小鼠

克隆类型

  • 95
  • 14
单克隆

标记

  • 51
  • 6
  • 6
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This ARAF antibody is un-conjugated

应用范围

  • 74
  • 41
  • 40
  • 39
  • 20
  • 18
  • 13
  • 9
  • 5
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

克隆位点

1-A
  • 抗原表位

    • 19
    • 18
    • 16
    • 15
    • 8
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 183-199

    交叉反应

    人, 小鸡, 犬, 大鼠

    产品特性

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

    纯化方法

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

    免疫原

    Mouse A-Raf aa. 183-199

    亚型

    IgG1
  • 说明

    Related Products: ABIN968533, ABIN967389

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    250 μg/mL

    缓冲液

    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store undiluted at -20° C.
  • Gomez, Pritchard, Herbert: "cAMP-dependent protein kinase and Ca2+ influx through L-type voltage-gated calcium channels mediate Raf-independent activation of extracellular regulated kinase in response to glucagon-like peptide-1 in pancreatic beta-cells." in: The Journal of biological chemistry, Vol. 277, Issue 50, pp. 48146-51, (2002) (PubMed).

    Storm, Cleveland, Rapp: "Expression of raf family proto-oncogenes in normal mouse tissues." in: Oncogene, Vol. 5, Issue 3, pp. 345-51, (1990) (PubMed).

    Rapp, Heidecker, Huleihel, Cleveland, Choi, Pawson, Ihle, Anderson: "raf family serine/threonine protein kinases in mitogen signal transduction." in: Cold Spring Harbor symposia on quantitative biology, Vol. 53 Pt 1, pp. 173-84, (1989) (PubMed).

    Huleihel, Goldsborough, Cleveland, Gunnell, Bonner, Rapp: "Characterization of murine A-raf, a new oncogene related to the v-raf oncogene." in: Molecular and cellular biology, Vol. 6, Issue 7, pp. 2655-62, (1987) (PubMed).

  • 抗原

    ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

    别名

    A-Raf

    背景

    A-Raf, a proto-oncogene that encodes a 68 kDa serine/threonine-specific protein kinase, shows strong homology to c-Raf and v-Raf in the kinase region of the molecule. c-Raf appears to be uniformly and ubiquitously expressed, but A-Raf expression is much more variable. A-Raf is found at highest levels in urogenital and kidney tissues, with the lowest levels found in brain. Cell transformation by A-Raf has been shown to be independent of p21[ras], suggesting the possibility of an independent signal transduction pathway. This antibody is routinely tested by western blot analysis.

    分子量

    68 kDa

    途径

    MAPK Pathway, RTK signaling, Hepatitis C
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