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VEGFR2/CD309 抗体

Name: VEGFR2/CD309 抗体
SKU: ABIN967481
Availability: OutOfStock

This anti-VEGFR2/CD309 antibody is a 大鼠单克隆 antibody detecting VEGFR2/CD309 in WB, IHC 和 FACS. Suitable for 小鼠. This Primary Antibody has been cited in 7+ publications.

(7 references)

产品编号 ABIN967481

发货至: 中国

Quick Overview for VEGFR2/CD309 抗体 (ABIN967481)

抗原

See all VEGFR2/CD309 (VEGFR2) 抗体

VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

适用

小鼠

宿主

大鼠

克隆类型

单克隆

标记

This VEGFR2/CD309 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

克隆位点

Avas 12alpha1

Discover our top product VEGFR2 Primary Antibody

品牌

BD Pharmingen™

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Mouse Flk1 and human IgG1 fusion protein

亚型

IgG2a kappa
anti-VEGF Receptor 2 (VEGFR2) (AA 20-200) antibody
VEGFR2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-VEGF Receptor 2 (VEGFR2) (pTyr951) antibody
VEGFR2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-VEGF Receptor 2 (VEGFR2) (AA 20-242) antibody
VEGFR2 适用: 人 WB, IHC, FACS, ICC 宿主: 兔 Polyclonal unconjugated

anti-VEGF Receptor 2 (VEGFR2) (AA 1326-1356) antibody
VEGFR2 适用: 人 WB, IHC, ELISA, FACS, IF 宿主: 兔 Polyclonal unconjugated

anti-VEGF Receptor 2 (VEGFR2) (AA 101-200) antibody
VEGFR2 适用: 人, 小鼠, 大鼠, 兔 ELISA, FACS, IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-VEGF Receptor 2 (VEGFR2) (pTyr1214) antibody
VEGFR2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-VEGF Receptor 2 (VEGFR2) (AA 20-764) antibody
VEGFR2 适用: 人 ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 4B4 unconjugated

anti-VEGF Receptor 2 (VEGFR2) (AA 1225-1356) antibody
VEGFR2 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 1A9A3 unconjugated

anti-VEGF Receptor 2 (VEGFR2) (AA 454-469), (Middle Region) antibody
VEGFR2 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-VEGF Receptor 2 (VEGFR2) (AA 1326-1356), (C-Term) antibody
VEGFR2 适用: 人 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB01536 unconjugated

应用备注

For immunohistochemistry application, we recommend to use purified Avas 12alpha1 mAb in our special formulation for immunohistochemistry.

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4° C.
Kataoka, Takakura, Nishikawa, Tsuchida, Kodama, Kunisada, Risau, Kita, Nishikawa: "Expressions of PDGF receptor alpha, c-Kit and Flk1 genes clustering in mouse chromosome 5 define distinct subsets of nascent mesodermal cells." in: Development, growth & differentiation, Vol. 39, Issue 6, pp. 729-40, (1998) (PubMed).

Nishikawa, Nishikawa, Hirashima, Matsuyoshi, Kodama: "Progressive lineage analysis by cell sorting and culture identifies FLK1+VE-cadherin+ cells at a diverging point of endothelial and hemopoietic lineages." in: Development (Cambridge, England), Vol. 125, Issue 9, pp. 1747-57, (1998) (PubMed).

Nishikawa, Nishikawa, Kawamoto, Yoshida, Kizumoto, Kataoka, Katsura: "In vitro generation of lymphohematopoietic cells from endothelial cells purified from murine embryos." in: Immunity, Vol. 8, Issue 6, pp. 761-9, (1998) (PubMed).

Hanahan: "Signaling vascular morphogenesis and maintenance." in: Science (New York, N.Y.), Vol. 277, Issue 5322, pp. 48-50, (1997) (PubMed).

Shalaby, Ho, Stanford, Fischer, Schuh, Schwartz, Bernstein, Rossant: "A requirement for Flk1 in primitive and definitive hematopoiesis and vasculogenesis." in: Cell, Vol. 89, Issue 6, pp. 981-90, (1997) (PubMed).

Shalaby, Rossant, Yamaguchi, Gertsenstein, Wu, Breitman, Schuh: "Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice." in: Nature, Vol. 376, Issue 6535, pp. 62-6, (1995) (PubMed).

Millauer, Wizigmann-Voos, Schnürch, Martinez, Møller, Risau, Ullrich: "High affinity VEGF binding and developmental expression suggest Flk-1 as a major regulator of vasculogenesis and angiogenesis." in: Cell, Vol. 72, Issue 6, pp. 835-46, (1993) (PubMed).
抗原

VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

别名

Flk1

背景

The Avas 12alpha1 antibody reacts with fetal liver kinase 1 (Flk-1), a receptor protein tyrosine kinase closely related to CD117 (c-kit) and CD140a (PDGF Receptor alpha chain) of the immunoglobulin superfamily. Flk-1, also known as VEGF Receptor-2 (VEGF-R2), is a receptor for vascular endothelial growth factor (VEGF). It is expressed, at the mRNA and protein levels, on distinct sets of mesoderm during gastrulation and on endothelial cells in embryonic tissues. In vivo and in vitro studies indicate that Flk-1 is required for the embryonic development of vascular endothelial and hematopoietic cells.
Synonyms: VEGF-R2, Ly-73

途径

RTK signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling