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CD25 抗体

There are 3+ publications for this product available. The 小鼠 单克隆 anti-CD25 antibody is suitable to detect CD25 in samples from 人. It has been validated for FACS, IHC (fro) 和 IHC (af).
产品编号 ABIN965780
发货至: 中国
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Quick Overview for CD25 抗体 (ABIN965780)

抗原

See all CD25 (IL2RA) 抗体
CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

适用

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小鼠

克隆类型

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单克隆

标记

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This CD25 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Acetone-fixed) (IHC (af))

克隆位点

B489 (143-13)
  • 特异性

    This antibody reacts a 55kD single chain glycoprotein, CD25, that associates with CD12 and CD132 to form the IL-2 receptor complex. CD25 has three epitope regions - A, B, and C - the latter of which is recognized by B489. This MAb has been clustered at the Fourth International Workshop on human differentiation antigens.

    纯化方法

    Concentrated.

    免疫原

    Human leucocytes.

    亚型

    IgG1
  • 应用备注

    Immunohistochemistry (acetone fixed frozen tissue): 1:50-1:100
    Flow cytometry: 1:150-1:300.
    Staining Procedure: This antibody can be used on frozen tissue sections (acetone fixed). The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

    说明

    Recommended Positive Control: Tonsil, Thymus, Spleen

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.3 mg/ml.

    缓冲液

    20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C
  • Parke: "Intravenous gammaglobulin in pregnancy, the Connecticut experience." in: Scandinavian journal of rheumatology. Supplement, Vol. 107, pp. 103-4, (1998) (PubMed).

    Goldstein, Marcon, Cullen, Nelson: "A competitive enzyme-linked immunoassay (ELISA) for the measurement of soluble human interleukin-2 receptors (IL-2R, Tac protein)." in: Journal of immunological methods, Vol. 107, Issue 1, pp. 103-9, (1988) (PubMed).

    Uchiyama, Nelson, Fleisher, Waldmann: "A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. II. Expression of Tac antigen on activated cytotoxic killer T cells, suppressor cells, and on one of two types of helper T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 4, pp. 1398-403, (1981) (PubMed).

  • 抗原

    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

    别名

    CD25 (IL-2R)

    基因ID

    3559

    途径

    JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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