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POLB 抗体 (C-Term)

There are 2+ publications for this product available. The 兔 多克隆 anti-POLB antibody is suitable to detect POLB in samples from 人. It has been validated for WB 和 ELISA.
Rockland
产品编号 ABIN964928
Supplier Product No.: 600-401-c65
发货至: 中国
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Quick Overview for POLB 抗体 (C-Term) (ABIN964928)

抗原

See all POLB 抗体
POLB (Polymerase (DNA Directed), beta (POLB))

适用

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宿主

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克隆类型

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多克隆

标记

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This POLB antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA
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    C-Term

    Supplier Product No.

    600-401-c65

    Supplier

    Rockland

    原理

    POLB Antibody

    交叉反应 (详细)

    This antibody reacts with overexpressed and endogenous POLß protein.

    产品特性

    Synonyms: rabbit anti-POLB antibody, rabbit anti-DNA polymerase beta antibody, DNA polymerase ß, POLß, POL-B, POL-ß, pol-beta, beta-pol

    纯化方法

    This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

    过滤

    Sterile filtered

    免疫原

    Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of the POLß protein.

    Immunogen Type: Conjugated Peptide

    亚型

    IgG
  • 应用备注

    Application Note: This affinity purified POLß antibody has been tested by ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38.1 kDa in size corresponding to POLß by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 0.5 μg/mL

    ELISA Dilution: 1:50,000

    Other: User Optimized

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1.4 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • Asagoshi, Liu, Masaoka, Lan, Prasad, Horton, Brown, Wang, Bdour, Sobol, Taylor, Yasui, Wilson: "DNA polymerase beta-dependent long patch base excision repair in living cells." in: DNA repair, Vol. 9, Issue 2, pp. 109-19, (2010) (PubMed).

    Sobol, Foley, Nyska, Davidson, Wilson: "Regulated over-expression of DNA polymerase beta mediates early onset cataract in mice." in: DNA repair, Vol. 2, Issue 5, pp. 609-22, (2004) (PubMed).

  • 抗原

    POLB (Polymerase (DNA Directed), beta (POLB))

    别名

    POLB

    背景

    Background: The POLß protein belongs to the DNA polymerase type-X family. It is a DNA polymerase involved in gap-filling DNA synthesis or base excision and repair in a stepwise distributive fashion unlike other DNA polymerases. It is required for DNA maintenance, replication, recombination, and drug resistance. It is normally found as a monomer, in the cytoplasm and it translocates to the nucleus upon DNA damage.

    基因ID

    5423

    NCBI登录号

    NP_002681

    UniProt

    P06746

    途径

    DNA Damage Repair
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