POLB 抗体 (C-Term)
(2 references)
Quick Overview for POLB 抗体 (C-Term) (ABIN964928)
抗原
See all POLB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-401-c65
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Supplier
- Rockland
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原理
- POLB Antibody
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交叉反应 （详细）
- This antibody reacts with overexpressed and endogenous POLß protein.
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产品特性
- Synonyms: rabbit anti-POLB antibody, rabbit anti-DNA polymerase beta antibody, DNA polymerase ß, POLß, POL-B, POL-ß, pol-beta, beta-pol
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纯化方法
- This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of the POLß protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Polymerase (DNA Directed), beta (POLB) (AA 1-335) antibody
POLB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), beta (POLB) (C-Term) antibodyPOLB 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), beta (POLB) (AA 1-335) antibodyPOLB 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Polymerase (DNA Directed), beta (POLB) (AA 1-300) antibodyPOLB 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), beta (POLB) (AA 55-160) antibodyPOLB 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), beta (POLB) (C-Term) antibodyPOLB 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), beta (POLB) (C-Term) antibodyPOLB 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), beta (POLB) (AA 224-333) antibodyPOLB 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Polymerase (DNA Directed), beta (POLB) (AA 1-335) antibodyPOLB 适用: 人 IP 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), beta (POLB) (AA 107-156) antibodyPOLB 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 犬, 山羊, 豚鼠, 马, 兔, Hamster, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity purified POLß antibody has been tested by ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38.1 kDa in size corresponding to POLß by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 0.5 μg/mL
ELISA Dilution: 1:50,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.4 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- POLB (Polymerase (DNA Directed), beta (POLB))
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别名
- POLB
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背景
- Background: The POLß protein belongs to the DNA polymerase type-X family. It is a DNA polymerase involved in gap-filling DNA synthesis or base excision and repair in a stepwise distributive fashion unlike other DNA polymerases. It is required for DNA maintenance, replication, recombination, and drug resistance. It is normally found as a monomer, in the cytoplasm and it translocates to the nucleus upon DNA damage.
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基因ID
- 5423
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NCBI登录号
- NP_002681
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UniProt
- P06746
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途径
- DNA Damage Repair
抗原
-
