IL-8 抗体
Quick Overview for IL-8 抗体 (ABIN964737)
抗原
See all IL-8 (IL8) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 202-401-311
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Supplier
- Rockland
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原理
- IL-8 Antibody
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交叉反应 (详细)
- This antibody is specific for cat IL-8 protein.
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产品特性
- Synonyms: IL8, rabbit anti-IL-8 antibody, rabbit anti-Interleukin-8 antibody, C-X-C motif chemokine 8, CXCL8, Chemokine (C-X-C motif) ligand 8
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纯化方法
- This product was protein A purified from monospecific antiserum by chromatography.
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免疫原
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Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to 79 amino acids of mature cat IL-8 protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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应用备注
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Application Note: This protein A purified CXCL8 antibody has been tested by Western blotting and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 8.9 kDa in size corresponding to cat IL-8 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500-1:2,000
ELISA Dilution: 1:10,000 - 1:50,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.13 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- IL-8 (IL8) (Interleukin 8 (IL8))
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别名
- CXCL8
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背景
- Background: IL-8 (CXCL8) is a small cytokine belonging to the chemokine CXC subfamily. There have been 17 different CXC chemokines described in mammals. The members of the CXC chemokine subfamily have been subdivided into two categories - those with a specific amino acid sequence of glutamic acid-leucine-arginine (ELR) immediately before the first cysteine of the CXC motif (ELR-positive), and those without an ELR motif (ELR-negative). ELR-positive CXC chemokines specifically induce the migration of neutrophils, and interact with chemokine receptors CXCR1 and CXCR2. IL-8 is an ELR-positive CXC chemokine. The primary function of IL-8 is to recruit neutrophils to the site of inflammation. It also activates neutrophil functions and promotes angiogenesis. Anti-IL8 antibody is ideal for investigators involved in Cancer, Cardiovascular and Immunology research.
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基因ID
- 493836
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UniProt
- Q9XSX5
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途径
- TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy
抗原
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