YBX1 抗体
-
- 抗原 See all YBX1 抗体
- YBX1 (Y Box Binding Protein 1 (YBX1))
-
适用
- Borrelia burgdorferi
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This YBX1 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 200-401-c16
- Supplier
- Rockland
- 原理
- DbpB Antibody
- 交叉反应 (详细)
- This antibody is specific for Borrelia burgdorferi DbpB protein.
- 产品特性
- Synonyms: rabbit anti-DbpB Antibody, Decorin-binding protein B, Borrelia burgdorferi DbpB, dbp-B, dbp B
- 纯化方法
- This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.
- 免疫原
-
Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi Dbp-B protein.
Immunogen Type: Recombinant Protein
- 亚型
- IgG
- Top Product
- Discover our top product YBX1 Primary Antibody
-
-
- 应用备注
-
Application Note: This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 17.9 kDa in size corresponding to Borrelia burgdorferi DbpB protein by Western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000
Other: User Optimized
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- YBX1 (Y Box Binding Protein 1 (YBX1))
- 别名
- DbpB (YBX1 产品)
- 背景
- Background: Decorin-binding protein B, or DbpB, binds to decorin, which may mediate the adherence of B.burgdorferi to collagen fibers in skin and other tissues. Spirochetal surface adhesions mediate attachment to decorin, a major component of the host extracellular matrix enabling bacteria to colonize in mammalian tissues. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH , and nutrients) and/or in coordination with the course of infection of the mammal host. Borrelia burgdorferi can colonize multiple tissues, and is capable of attachment to diverse cell types. The expression of decorin-binding protein (Dbp) A and/or DbpB, two B. burgdorferi surface proteins that bind GAGs, is sufficient to convert a high-passage nonadherent B. burgdorferi strain into one that efficiently binds 293 epithelial cells.
- 基因ID
- 1194341
- NCBI登录号
- WP_010890381
- UniProt
- P0CL68
- 途径
- Regulation of Muscle Cell Differentiation
-