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beta Actin 抗体 (AA 350-375) (HRP)

Name: beta Actin 抗体 (AA 350-375) (HRP)
SKU: ABIN964577
Availability: OutOfStock

Rockland 600-403-886 ACTB 适用: 人, 小鼠, 大鼠, 猴, Pig, Cow, 绵羊, Hamster, 豚鼠, 山羊 WB, ELISA, IHC 宿主: 兔 Polyclonal HRP

(1 reference)

产品编号 ABIN964577

Supplier Product No.: 600-403-886

发货至: 中国

Datasheet as PDF

抗原 See all beta Actin (ACTB) 抗体

beta Actin (ACTB) (Actin, beta (ACTB))
抗原表位
41

16

15

6

6

4

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 350-375
适用
177

130

110

55

42

41

36

26

25

23

17

16

11

10

8

7

6

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1
人, 小鼠, 大鼠, 猴, Pig, Cow, 绵羊, Hamster, 豚鼠, 山羊
宿主
112

81

5

1

1
兔
Compatible Secondaries
克隆类型
111

87
多克隆
标记
127

12

11

10

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This beta Actin antibody is conjugated to HRP
应用范围
167

73

51

46

33

31

26

21

13

13

8

6

6

6

5

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.

600-403-886

Supplier

Rockland

原理

Beta Actin Antibody Peroxidase Conjugated

交叉反应（详细）

Beta Actin Antibody Peroxidase Conjugated antibody is directed against human beta Actin protein.

产品特性

Synonyms: rabbit anti-beta Actin Antibody HRP conjugation, peroxidase conjugated rabbit anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody

纯化方法

The product was affinity purified from monospecific antiserum by immunoaffinity purification.

免疫原

Optional[Immunogen]: beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 350-375 of Human beta Actin.

亚型

IgG

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anti-Actin, beta (ACTB) (AA 2-16) antibody
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anti-Actin, beta (ACTB) (AA 350-375) antibody
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anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
KO Validated ACTB 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB 适用: 人, 小鼠, 小鸡, 兔, Pig, Cow, Hamster, 山羊, 鱼 WB, ELISA 宿主: 小鼠 Monoclonal 2D1D10 unconjugated

anti-Actin, beta (ACTB) antibody
ACTB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), FACS, DNA Mic 宿主: 小鼠 Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB 适用: 人 WB, ELISA, IHC, IF, IP, FACS 宿主: 小鼠 Monoclonal 1D12E12B5 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
ACTB 适用: 人, 小鼠, 大鼠, 小鸡, 兔, 犬, 猴, Pig, Cow, 绵羊, Hamster, 马, 豚鼠, 山羊, Cat, Avian, 驴 WB, IF 宿主: 山羊 Polyclonal unconjugated

应用备注

Immunohistochemistry Dilution: 1:500 - 1:2,500
Application Note: Beta Actin Antibody Peroxidase Conjugated antibody has been tested for use in ELISA and is suitable in immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:20,000
Other: User Optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

储存液

Gentamicin sulfate

注意事项

This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Xie, Collins: "Copper stabilizes the Menkes copper-transporting ATPase (Atp7a) protein expressed in rat intestinal epithelial cells." in: American journal of physiology. Cell physiology, Vol. 304, Issue 3, pp. C257-62, (2013) (PubMed).
抗原

beta Actin (ACTB) (Actin, beta (ACTB))

别名

ACTB (ACTB 产品)

背景

Background: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Beta-Actin Peroxidase conjugate Antibody is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.

UniProt

P60709

途径

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction