Caspase 2 抗体 (N-Term)
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Quick Overview for Caspase 2 抗体 (N-Term) (ABIN96091)
抗原
See all Caspase 2 (CASP2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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Supplier Product No.
- 600-401-412
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Supplier
- Rockland
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原理
- Caspase-2 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against rat and mouse caspase-2. The antibody detects long and short forms of the protein.
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产品特性
- Synonyms: rabbit anti-Caspase-2 Antibody, Apoptosis related cysteine peptidase antibody, CASP-2, CASP 2 antibody, Casp 2L Pro antibody, CASP2 antibody, Caspase 2 apoptosis related cysteine protease antibody, Caspase2 antibody, Caspase-2, Developmentally down regulated 2 antibody, ICH 1 antibody, ICH-1, Caspase-2 subunit p18, Caspase-2 subunit p13, Caspase-2 subunit p12
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纯化方法
- Affinity purified antibody
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过滤
- Sterile filtered
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免疫原
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Immunogen: A synthetic peptide corresponding to the N-terminus of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) (AA 334-452) antibody
CASP2 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) (AA 153-452) antibodyCASP2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) antibodyCASP2 适用: 人 WB, ELISA, IHC 宿主: 兔 Monoclonal 3D4 unconjugated Recombinant Antibody
anti-Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) antibodyKD Validated CASP2 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB3305 unconjugated Recombinant Antibody
anti-Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) antibodyKD Validated CASP2 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB4375 unconjugated Recombinant Antibody
anti-Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) (AA 417-439), (C-Term) antibodyCASP2 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) (cleaved), (Internal Region) antibodyCASP2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) (AA 2-452) antibodyCASP2 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) (AA 121-220) antibodyCASP2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) (Internal Region) antibodyCASP2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity purified polyclonal antibody was tested by ELISA and immunoblotting and was found to be reactive with 48 kDa mouse and rat caspase-2. A control and rat caspase-2 transfected human 293 cell lysate was used to show by immunoblotting that this antibody detects rat caspase-2. No reaction was observed against human caspase-2. Immunoblot of mouse neuronal cell lysates from wild-type and caspase-2 (-/-) mice (not shown) was used to show that this antibody cross reacts with mouse caspase-2. A 1:750 dilution is recommended for immunoblot. This product was assayed against 1.0 μg of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product.
Western Blot Dilution: 1:750
ELISA Dilution: 1:1,500 - 1:6,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.44 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))
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别名
- Casp2
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背景
- Background: Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer and other diseases. Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. Caspase-2 has been called ICH-1 in human, CED-3 in C.elegans and NEDD-2 in mouse. Caspase-2 is synthesized as an inactive precursor that is processed in cells undergoing apoptosis. The precursor form of all caspases is composed of a pro-domain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli, including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. Caspase-2 is activated in vitro by caspase-1, caspase-3 and granzyme B. Caspase-2 functions as an upstream apoptosis initiator that participates in the activation of downstream caspases, such as caspase-3, caspase-6 and caspase-7. Caspase-2 is highly expressed in embryonic mouse brain but not in adult neural tissue. Alternative mRNA splicing produces a long (48 kDa) and short (35 kDa) form of caspase-2. These forms show tissue specificity and function either as inducers or suppressors of apoptosis. Mature caspase-2 is 435 amino acids in length cleaved (~50 kDa) and is cleaved upon activation into 10 and 20 kDa subunits.
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基因ID
- 64314
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UniProt
- P55215
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途径
- Apoptosis, Caspase Cascade in Apoptosis, Neurotrophin Signaling Pathway
抗原
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