ASK1 抗体 (pSer83)
(1 reference)
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Quick Overview for ASK1 抗体 (pSer83) (ABIN95283)
抗原
See all ASK1 (MAP3K5) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer83
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Supplier Product No.
- 200-401-410
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Supplier
- Rockland
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原理
- ASK1 phospho S83 Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
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产品特性
- Synonyms: rabbit anti-ASK1 pS83 Antibody, Apoptosis signal regulating kinase 1 antibody, ASK 1 antibody, ASK-1 antibody, MAP/ERK kinase kinase 5 antibody, MAP3K5 antibody, MAPK/ERK kinase kinase 5 antibody
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纯化方法
- This product is an IgG fraction antibody purified from antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with a KLH conjugated peptide corresponding to an internal region near amino acids 75-100 of human ASK-1 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (pThr845) antibody
MAP3K5 适用: 人, 小鼠, 大鼠, Cow WB, ELISA, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) antibodyMAP3K5 适用: 人 IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 2E4 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (pSer966) antibodyMAP3K5 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1231-1374) antibodyMAP3K5 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2D11 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 693-933) antibodyMAP3K5 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 821-849) antibodyMAP3K5 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB12839 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (pSer83) antibodyMAP3K5 适用: 人 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (N-Term) antibodyMAP3K5 适用: 人, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (C-Term) antibodyMAP3K5 适用: 人, 小鼠 WB, IHC, ELISA, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (AA 1231-1374) antibodyMAP3K5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1E3 unconjugated
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应用备注
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Application Note: This phospho specific polyclonal antibody reacts human pS83 ASK1 and shows minimal reactivity by western blot, ELISA and competitive ELISA with non-phosphorylated ASK1. Although not tested, this antibody is likely functional in RIA, immunohistochemistry and immunoprecipitation. For immunoblotting a 1:1,000 dilution is recommended. A 155 kDa band corresponding to human ASK-1 is detected. Whole cell lysates from SW1353 can be used as a positive control. For ELISA a 1:5,000 to 1:10,000 dilution is recommended. Researchers should determine optimal titers for other applications.
Western Blot Dilution: 1:1,000
ELISA Dilution: 1:5,000 - 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Inhibition of protein kinase Akt1 by apoptosis signal-regulating kinase-1 (ASK1) is involved in apoptotic inhibition of regulatory volume increase." in: The Journal of biological chemistry, Vol. 285, Issue 9, pp. 6109-17, (2010) (PubMed).
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: "Inhibition of protein kinase Akt1 by apoptosis signal-regulating kinase-1 (ASK1) is involved in apoptotic inhibition of regulatory volume increase." in: The Journal of biological chemistry, Vol. 285, Issue 9, pp. 6109-17, (2010) (PubMed).
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- ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
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别名
- MAP3K5
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背景
- Background: ASK-1 (apoptosis signal-regulating kinase 1 - also referred to as MEK Kinase-5 or MAPKKK5) is a novel serine/threonine MAP kinase kinase kinase (MAPKKK) component of the mitogen -activated protein (MAP) cascade that is activated in response to extracellular stimuli by cytokines, growth factors and environmental stresses and other factors. Overexpression of ASK-1 induces apoptotic cell death. ASK-1 is expressed in a variety of human and mouse tissues. The overall amino acid sequence identity between the mouse and human ASK1 is 91.9 % . ASK-1 interacts with CDKN1A (also known as p21, WAF1, CIP1). Please refer to the reference list at the end of this document for further information.
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基因ID
- 4217
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NCBI登录号
- NP_005914
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UniProt
- Q99683
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途径
- MAPK Pathway, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
抗原
-
