RELB 抗体 (AA 1-579)
(1 validation)-
- 抗原 See all RELB 抗体
- RELB (V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB))
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抗原表位
- AA 1-579
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 多克隆
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标记
- This RELB antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 原理
- Mouse polyclonal antibody raised against a full-length human RELB protein.
- 序列
- MLRSGPASGP SVPTGRAMPS RRVARPPAAP ELGALGSPDL SSLSLAVSRS TDELEIIDEY IKENGFGLDG GQPGPGEGLP RLVSRGAASL STVTLGPVAP PATPPPWGCP LGRLVSPAPG PGPQPHLVIT EQPKQRGMRF RYECEGRSAG SILGESSTEA SKTLPAIELR DCGGLREVEV TACLVWKDWP HRVHPHSLVG KDCTDGICRV RLRPHVSPRH SFNNLGIQCV RKKEIEAAIE RKIQLGIDPY NAGSLKNHQE VDMNVVRICF QASYRDQQGQ MRRMDPVLSE PVYDKKSTNT SELRICRINK ESGPCTGGEE LYLLCDKVQK EDISVVFSRA SWEGRADFSQ ADVHRQIAIV FKTPPYEDLE IVEPVTVNVF LQRLTDGVCS EPLPFTYLPR DHDSYGVDKK RKRGMPDVLG ELNSSDPHGI ESKRRKKKPA ILDHFLPNHG SGPFLPPSAL LPDPDFFSGT VSLPGLEPPG GPDLLDDGFA YDPTAPTLFT MLDLLPPAPP HASAVVCSGG AGAVVGETPG PEPLTLDSYQ APGPGDGGTA SLVGSNMFPN HYREAAFGGG LLSPGPEAT
- 交叉反应
- 人
- 产品特性
- Antibody reactive against mammalian transfected lysate.
- 免疫原
- RELB (NP_006500.2, 1 a.a. ~ 579 a.a) full-length human protein.
- Top Product
- Discover our top product RELB Primary Antibody
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anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) (AA 1-579) antibody
RELB 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) (C-Term) antibodyRELB 适用: 人, 小鼠 WB, IP, IHC (p), ChIP 宿主: 兔 Polyclonal unconjugated
anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) (pSer573) antibodyRELB 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) (AA 248-492) antibodyRELB 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) (AA 161-260) antibodyRELB 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) antibodyRELB 适用: 人, 小鼠 WB 宿主: 小鼠 Monoclonal 4C5 unconjugated
anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) (AA 400-579) antibodyRELB 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) (pSer573) antibodyRELB 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) antibodyRELB 适用: 人, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) (N-Term) antibodyRELB 适用: 人, 犬, 豚鼠, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- by
- Klinik für Anästhesiologie, Intensivmedizin und Schmerztherapie des Universitätsklinikums Knappschaftskrankenhaus Bochum
- No.
- #104077
- 日期
- 2019.11.21
- 抗原
- RELB
- Lot Number
- 10307
- Method validated
- Immunofluorescence
- Positive Control
U-937 human monocytic cell line
- Negative Control
No primary antibody control
- Notes
Passed. ABIN948396 specifically stains RelB in U-937 human monocytic cells.
- Primary Antibody
- ABIN948396
- Secondary Antibody
- AF488-conjugated AffiniPure alpaca anti-mouse IgG antibody (Jackson Immuno Research, 615-545-214)
- Full Protocol
- Grow U-937 cells in RPMI 1640 Medium (Gibco, 21875034) supplemented with 10 % FCS (Gibco, A3840001) and Pen/Strep (Gibco, 15140122), at 37°C and 5% CO2 in 300µL in 24-well plates (Sarstedt AG&Co. KG).
- Use cytospin to spin cells onto slide.
- Fix cells on coverslide in 4% paraformaldehyde (Carl Roth GmbH, 0335.1) for 30min at RT.
- Wash cells 3x for 5min with PBS (Sigma-Aldrich, 8537).
- Permeabilize cells in 100µL PBS containing 0.1 % Triton X-100 (Sigma-Aldrich, T8787) for 5min at RT.
- Wash cells 3x for 5min with PBS.
- Block non-specific binding with 1x Duolink Blocking solution (Sigma-Aldrich, DUO82007) for 30min at RT.
- Incubate cells with primary mouse anti-human RelB antibody (antibodies-online, ABIN948396, lot 10307) diluted 1:50 in 1x Duolink Antibody diluent (Sigma-Aldrich, DUO82008) ON at 4°C.
- Wash cells 3x for 5min with PBS.
- Incubate cells with secondary AF488-conjugated AffiniPure alpaca anti-mouse IgG antibody (Jackson Immuno Research, 615-545-214) diluted 1:400 in 1x Duolink Antibody diluent for 1h at RT in the dark.
- Wash cells 2x for 5min with PBS.
- Mount coverslips on glass slides in 50µL ProLong Gold antifade mountant with DAPI (Invitrogen, P36931).
- Image acquisition with an Olympus widefield microscope.
- Experimental Notes
Strong green staining dependent on the presence of ABIN948396 indicates that the antibody works. Negative control lacking the primary antibody shows only background fluorescence.
生效 #104077 (Immunofluorescence)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![U937 monocytic cells stained with with anti-RelB antibody ABIN948396 and an AF488-labeled secondary antibody (bottom row, green). Counterstain with DAPI (blue). Negative control without primary antibody (top row) shows no AF488 signal.]()
U937 monocytic cells stained with with anti-RelB antibody ABIN948396 and an AF488-labeled secondary antibody (bottom row, green). Counterstain with DAPI (blue). Negative control without primary antibody (top row) shows no AF488 signal.
Full Methods -
- 缓冲液
- In 1x PBS, pH 7.4
- 注意事项
- Aliquot to avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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- 抗原
- RELB (V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB))
- 别名
- RELB (RELB 产品)
- 别名
- shep antibody, I-REL antibody, IREL antibody, REL-B antibody, XRelB antibody, relb-A antibody, GC-rich antibody, avian reticuloendotheliosis viral (v-rel) oncogene related B antibody, RELB proto-oncogene, NF-kB subunit antibody, v-rel avian reticuloendotheliosis viral oncogene homolog B S homeolog antibody, Relb antibody, RELB antibody, relb.S antibody
- 背景
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Full Gene Name: v-rel reticuloendotheliosis viral oncogene homolog B
Synonyms: I-REL,IREL - 基因ID
- 5971
- NCBI登录号
- NM_006509
- 途径
- NF-kappaB Signaling, RTK signaling
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