PAG1 抗体 (AA 97-432)
(5 references)Quick Overview for PAG1 抗体 (AA 97-432) (ABIN94272)
抗原
See all PAG1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 97-432
-
原理
- Anti-Hu PAG1 Purified
-
特异性
- The antibody MEM-255 recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG).
-
无交叉反应
- Cow, 小鼠, 大鼠
-
交叉反应 （详细）
- Human
-
纯化方法
- Purified by protein-A affinity chromatography.
-
纯度
- > 95 % (by SDS-PAGE)
-
免疫原
- Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG).
-
亚型
- IgG2a
-
-
anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (AA 97-432) antibody
PAG1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (C-Term) antibodyPAG1 适用: 人, 小鼠, 大鼠, Cow WB, IP, IHC (p) 宿主: 小鼠 Monoclonal PAG-C1 unconjugated
anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (AA 331-432) antibodyPAG1 适用: 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (AA 97-432) antibodyPAG1 适用: 人, 小鼠 WB, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (AA 160-390) antibodyPAG1 适用: 小鼠 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (AA 138-357) antibodyPAG1 适用: 大鼠 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (AA 54-380) antibodyPAG1 适用: Cow WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (AA 250-350) antibodyPAG1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Flow cytometry: Recommended dilution: 2 μg/mL. Intracellular staining.
Immunohistochemistry (paraffin sections): Positive tissue: tonsil, spleen.
Western blotting: Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which, however, migrates on SDS-PAGE gels anomalously as an 80 kDa molecule. -
限制
- 仅限研究用
-
-
-
浓度
- 1 mg/mL
-
缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Do not freeze.
-
储存条件
- 4 °C
-
储存方法
- Store at 2-8°C. Do not freeze.
-
-
-
: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).
: "The sphingolipid-rich rafts of ALK+ lymphomas downregulate the Lyn-Cbp/PAG signalosome." in: European journal of haematology, (2010) (PubMed).
: "Transmembrane adaptor molecules: a new category of lymphoid-cell markers." in: Blood, Vol. 107, Issue 1, pp. 213-21, (2005) (PubMed).
: "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." in: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2017-28, (2003) (PubMed).
: "Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton." in: FEBS letters, Vol. 507, Issue 2, pp. 133-6, (2001) (PubMed).
-
: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).
-
- PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))
-
别名
- PAG1
-
背景
- Phosphoprotein membrane anchor with glycosphingoli,PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.,CBP, PAG
-
基因ID
- 55824
-
UniProt
- Q9NWQ8
-
途径
- p53 Pathway, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events
抗原
-
