PAG1 抗体 (C-Term)
(2 references)Quick Overview for PAG1 抗体 (C-Term) (ABIN94270)
抗原
See all PAG1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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原理
- Anti-PAG1 Purified
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特异性
- The antibody PAG-C1 recognizes an epitope located in the intracellular C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
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交叉反应 （详细）
- Mouse, Human, Rat, Bovine, Other not tested
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
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亚型
- IgG2b
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anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (AA 97-432) antibody
PAG1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (AA 97-432) antibodyPAG1 适用: 人 WB, IHC (p), ICFC 宿主: 小鼠 Monoclonal MEM-255 unconjugated
anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (AA 331-432) antibodyPAG1 适用: 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (AA 97-432) antibodyPAG1 适用: 人, 小鼠 WB, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (AA 160-390) antibodyPAG1 适用: 小鼠 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (AA 138-357) antibodyPAG1 适用: 大鼠 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (AA 54-380) antibodyPAG1 适用: Cow WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (AA 250-350) antibodyPAG1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry (paraffin sections): Positive tissue: appendix (germinal center of lymphatic follicle), heat-mediated antigen retrieval in citrate buffer pH 6.1.
Immunoprecipitation: Positive control: RAJI human Burkitt lymphoma cell line. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." in: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2017-28, (2003) (PubMed).
: "Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17597-600, (2003) (PubMed).
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: "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." in: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2017-28, (2003) (PubMed).
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- PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))
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别名
- PAG1
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背景
- Phosphoprotein membrane anchor with glycosphingoli,PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.,CBP, PAG
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基因ID
- 94212
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UniProt
- Q3U1F9
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途径
- p53 Pathway, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events
抗原
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