Integrin beta 2 抗体
(3 references)-
- 抗原 See all Integrin beta 2 (ITGB2) 抗体
- Integrin beta 2 (ITGB2)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Integrin beta 2 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)
- 特异性
- The antibody MEM-148 recognizes an extracellular epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18. CD18 (integrin beta2 subunit, beta2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
- 无交叉反应
- Pig
- 交叉反应 （详细）
- Human
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Peripheral blood mononuclear cells
- 克隆位点
- MEM-148
- 亚型
- IgG1
- Top Product
- Discover our top product ITGB2 Primary Antibody
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anti-Integrin beta 2 (ITGB2) antibody
ITGB2 适用: 人 FACS, WB, IP, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal MEM-48 unconjugated
anti-Integrin beta 2 (ITGB2) antibodyITGB2 适用: 人 FACS, WB, IP, IHC (fro), Func, IHC (p) 宿主: 小鼠 Monoclonal MEM-48 unconjugated
anti-Integrin beta 2 (ITGB2) antibody (FITC)ITGB2 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-48 FITC
anti-Integrin beta 2 (ITGB2) antibody (Biotin)ITGB2 适用: 人 FACS, WB, IP, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal MEM-48 Biotin
anti-Integrin beta 2 (ITGB2) antibody (PE)ITGB2 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-48 PE
anti-Integrin beta 2 (ITGB2) (AA 70-170) antibodyITGB2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 2 (ITGB2) antibodyITGB2 适用: 小鼠 FACS, WB, IP, IHC (fro), Func, IHC (p) 宿主: 大鼠 Monoclonal M18-2 unconjugated
anti-Integrin beta 2 (ITGB2) (AA 559-700) antibodyITGB2 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 3C11G5 unconjugated
anti-Integrin beta 2 (ITGB2) (AA 17-382) antibodyITGB2 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 2 (ITGB2) (AA 701-770) antibodyITGB2 适用: 人, 大鼠 ELISA, IHC (fro), IHC (p), ICC, IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Flow cytometry: Recommended dilution: 1-5 μg/mL. The antibody MEM-148 is an excellent marker of activated myeloid cells.
Western blotting: Non-reducing conditions. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, (2009) (PubMed).
: "A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins." in: Immunobiology, Vol. 203, Issue 4, pp. 687-98, (2001) (PubMed).
: "Human leukocytes contain a large pool of free forms of CD18." in: Biochemical and biophysical research communications, Vol. 275, Issue 2, pp. 295-9, (2000) (PubMed).
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: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, (2009) (PubMed).
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- 抗原
- Integrin beta 2 (ITGB2)
- 别名
- CD18 (ITGB2 产品)
- 背景
- Integrin subunit beta 2,CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.,Integrin beta2, Complement receptor C3 subunit beta, ITGB2
- 基因ID
- 3689
- UniProt
- P05107
- 途径
- NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
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