Integrin beta 2 抗体
(3 references)Our Local Distributor
北京 101111
Quick Overview for Integrin beta 2 抗体 (ABIN94010)
抗原
See all Integrin beta 2 (ITGB2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Anti-Hu CD18 Purified
-
特异性
- The antibody MEM-148 recognizes an extracellular epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18. CD18 (integrin beta2 subunit, beta2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
-
无交叉反应
- Pig
-
交叉反应 （详细）
- Human
-
纯化方法
- Purified by protein-A affinity chromatography.
-
纯度
- > 95 % (by SDS-PAGE)
-
免疫原
- Peripheral blood mononuclear cells
-
亚型
- IgG1
-
-
anti-Integrin beta 2 (ITGB2) antibody
ITGB2 适用: 人 FACS, WB, IP, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal MEM-48 unconjugated
anti-Integrin beta 2 (ITGB2) antibodyITGB2 适用: 人 FACS, WB, IP, Func, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal MEM-48 unconjugated
anti-Integrin beta 2 (ITGB2) (AA 70-170) antibodyITGB2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 2 (ITGB2) antibody (Biotin)ITGB2 适用: 人 FACS, WB, IP, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal MEM-48 Biotin
anti-Integrin beta 2 (ITGB2) antibody (FITC)ITGB2 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-48 FITC
anti-Integrin beta 2 (ITGB2) antibody (PE)ITGB2 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-48 PE
anti-Integrin beta 2 (ITGB2) antibodyITGB2 适用: 小鼠 FACS, WB, IP, Func, IHC (fro), IHC (p) 宿主: 大鼠 Monoclonal M18-2 unconjugated
anti-Integrin beta 2 (ITGB2) (AA 559-700) antibodyITGB2 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 3C11G5 unconjugated
anti-Integrin beta 2 (ITGB2) (AA 17-382) antibodyITGB2 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin beta 2 (ITGB2) (AA 701-770) antibodyITGB2 适用: 人, 大鼠 ELISA, IHC (fro), ICC, IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Flow cytometry: Recommended dilution: 1-5 μg/mL. The antibody MEM-148 is an excellent marker of activated myeloid cells.
Western blotting: Recommended dilution: 1-2 μg/mL. -
限制
- 仅限研究用
-
-
-
浓度
- 1 mg/mL
-
缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Do not freeze.
-
储存条件
- 4 °C
-
储存方法
- Store at 2-8°C. Do not freeze.
-
-
-
: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, (2009) (PubMed).
: "A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins." in: Immunobiology, Vol. 203, Issue 4, pp. 687-98, (2001) (PubMed).
: "Human leukocytes contain a large pool of free forms of CD18." in: Biochemical and biophysical research communications, Vol. 275, Issue 2, pp. 295-9, (2000) (PubMed).
-
: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, (2009) (PubMed).
-
- Integrin beta 2 (ITGB2)
-
别名
- CD18
-
背景
- Integrin subunit beta 2,CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.,Integrin beta2, Complement receptor C3 subunit beta, ITGB2
-
基因ID
- 3689
-
UniProt
- P05107
-
途径
- NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
抗原
-
