His Tag 抗体
Quick Overview for His Tag 抗体 (ABIN933979)
抗原
See all His Tag 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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序列
- HHHHHH
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纯化方法
- Protein A affinity chromatography
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免疫原
- His Tag antibody was raised in mouse using HHHHHH (6 x His) synthetic peptide conjugated to KLH as the immunogen.
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亚型
- IgG2b
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anti-His Tag antibody
WB, ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibody适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated
anti-His Tag antibodyWB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated
anti-His Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated
anti-His Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated
anti-His Tag antibody (Cy3)WB, ELISA, FACS, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 Cy3
anti-His Tag antibody (DyLight 800)WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 800
anti-His Tag antibody (DyLight 649)WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 649
anti-His Tag antibody (HRP)WB, ELISA, IHC 宿主: 小鼠 Monoclonal 33D10-D2-G8 HRP
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应用备注
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WB: 1:1000-3000
Optimal conditions should be determined by the investigator. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3
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储存液
- Sodium azide
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注意事项
- This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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注意事项
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. -
储存条件
- 4 °C/-20 °C
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储存方法
- Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.
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- His Tag
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物质类
- Tag
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背景
- A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. The tag was invented by Roche. His-tags are useful in a wide variety of processes including protein purification and binding assays. Synonyms: Monoclonal His Tag antibody, Anti-His Tag antibody, 6XHis antibody, Polyhistidine Tag antibody, HIS6 Tag antibody, 6-His antibody, HHHHHH antibody.
抗原
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