CDT2/RAMP 抗体 (AA 181-280)
Quick Overview for CDT2/RAMP 抗体 (AA 181-280) (ABIN873173)
抗原
See all CDT2/RAMP (DTL) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 181-280
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预测反应
- Human,Mouse,Rat,Dog,Sheep,Pig,Horse
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纯化方法
- Purified by Protein A.
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免疫原
- KLH conjugated synthetic peptide derived from human RAMP/CDT2
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亚型
- IgG
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anti-Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL) (AA 393-550) antibody
DTL 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL) (AA 1-730) antibodyDTL 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL) (AA 229-256), (Middle Region) antibodyDTL 适用: 人, 小鼠 WB, FACS, EIA 宿主: 兔 Polyclonal unconjugated
anti-Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL) (AA 182-231) antibodyDTL 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, Bat, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL) (AA 541-730) antibodyDTL 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL) (AA 229-256) antibodyDTL 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB24420 unconjugated
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应用备注
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
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有效期
- 12 months
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- CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))
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别名
- CDT2
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背景
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Synonyms: Lethal2 denticleless protein homolog, CDW1, CDW1, DCAF2, DDB1 and CUL4 associated factor 2, Ddb1- and Cul4-associated factor 2, Denticleless homolog, Denticleless homolog Drosophila, Denticleless protein homolog, Dtl, DTL_HUMAN, L2DTL, Lethal2 denticleless protein homolog, RA regulated nuclear matrix associated protein, RAMP, Retinoic acid regulated nuclear matrix associated protein, Retinoic acid-regulated nuclear matrix-associated protein.
Background: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.Tissue specificity: Expressed in placenta and testis, very low expression seen in skeletal muscle. Detected in all hematopoietic tissues examined, with highest expression in thymus and bone marrow. A low level detected in the spleen and lymph node, and barely detectable level in the peripheral leukocytes. RA treatment down-regulated the expression in NT2 cell.
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基因ID
- 51514
抗原
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