GAPDH 抗体 (AA 252-256)
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Quick Overview for GAPDH 抗体 (AA 252-256) (ABIN871714)
抗原
See all GAPDH 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 252-256
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特异性
- The antibody detects endogenous level of total GAPDH protein.
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纯化方法
- Affinity purified
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免疫原
- Peptide sequence around AA 252-256 (P-A-K-Y-D) derived from Human GAPDH. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
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应用备注
- Western blotting: 1:500-1:1000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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别名
- GAPDH
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背景
- Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
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分子量
- 37 kDa
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基因ID
- 2597
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NCBI登录号
- NP_002037
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UniProt
- P04406
抗原
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