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CD4 抗体

CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal 7E14 unconjugated
产品编号 ABIN793377
发货至: 中国
  • 抗原 See all CD4 抗体
    CD4
    适用
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    小鼠
    克隆类型
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    单克隆
    标记
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    This CD4 antibody is un-conjugated
    应用范围
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    Flow Cytometry (FACS)
    特异性
    Human CD4
    纯化方法
    Protein A/G purified
    免疫原
    Human peripheral blood T lymphocytes.
    克隆位点
    7E14
    亚型
    IgG1
    Top Product
    Discover our top product CD4 Primary Antibody
  • 应用备注
    Approved: Flo

    Usage: PBMC: Add 10 μL of antibody/10^6 PBMC in 100 μL PBS. Mix gently and incubate for 15 minutes at 2to 8 °C. Wash twice with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 μL of antibody/100 μL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20 °C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Excites at 488nm and emits at 670nm. Store protected from light.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS, 0.08 % Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Aliquot to Avoid freeze/thaw cycles.
    储存条件
    -20 °C
    储存方法
    Store at -20°C. Aliquot to avoid freeze/thaw cycles.
  • 抗原
    CD4
    别名
    CD4 (CD4 产品)
    背景
    Name/Gene ID: CD4

    Synonyms: CD4, CD4 antigen, CD4 receptor, CD4mut, CD4 antigen (p55), CD4 Molecule
    基因ID
    920
    UniProt
    P01730
    途径
    TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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