His Tag 抗体
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北京 101111
Quick Overview for His Tag 抗体 (ABIN781760)
抗原
See all His Tag 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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序列
- HHHHHH
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纯化方法
- Affinity Chromatography on Protein G
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免疫原
- Purified, recombinant C-terminal His6-tagged protein expressed in E. coli
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亚型
- IgG2b
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS pH 7.4, 15 mM Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Upon receipt, store (in aliquots) at -20 °C to -70 °C.
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- His Tag
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别名
- 6xHistidine Epitope Tag
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物质类
- Tag
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背景
- 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag
抗原
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