NRF2 抗体 (AA 445-458)
Quick Overview for NRF2 抗体 (AA 445-458) (ABIN768601)
抗原
See all NRF2 (NFE2L2) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 445-458
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原理
- NRF2 (aa445-458)
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序列
- KHSSRLEAHL TRDE
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特异性
- This antibody is expected to recognize all reported isoforms (
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交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-KHSSRLEAHLTRDE, from the internal region of the protein sequence according to NP_006155.2, NP_001138884.1, NP_001138885.1.
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亚型
- IgG
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应用备注
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Western Blot: Approx 70 kDa band observed in Human Lung lysates and approx 75 kDa in preliminary testing of Mouse and Rat Liver and KNRK, MDCK, and nuclear NIH3T3 cell lysate (calculated MW of 67.8 kDa according to NP_006155.2). This molecular weight rate
Peptide ELISA: antibody detection limit dilution 1:64000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS and HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended conc
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))
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别名
- NFE2L2
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背景
- NFE2L2, nuclear factor (erythroid-derived 2)-like 2, NRF2, HEBP1, NF-E2-related factor 2, NFE2-related factor 2, OTTHUMP00000205251, OTTHUMP00000207980, nuclear factor erythroid 2-related factor 2, nuclear factor, erythroid derived 2, like 2
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分子量
- 67.8kDa according to NP_006155.2
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基因ID
- 4780, 18024, 83619
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NCBI登录号
- NP_006155, NP_001138884, NP_001138885
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途径
- ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling
抗原
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