我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

NRF2 抗体 (Internal Region)

Name: NRF2 抗体 (Internal Region)
Brand: antibodies-online
SKU: ABIN2855612
Availability: OutOfStock

NFE2L2 适用: 人, 大鼠 WB, IF, ICC, IP, IHC (p), ChIP 宿主: 兔 Polyclonal unconjugated

WB Image NRF2 antibody detects NRF2 protein by western blot analysis.

ICC/IF Image NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NRF2, antibody at 1:500 dilution.

IHC

WB Image Untreated (–) and treated (+) MDA-MB-231 nuclear extracts (30 μg) were separated by 7.

ChIP

IP Image Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 μg of NRF2 antibody [N2C2], Internal, Western blot analysis was performed using NRF2 antibody [N2C2], Internal, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Nuclear factor erythroid-derived 2-like 2 (NRF2) protein expression in nuclear extracts of PBMCs from healthy subjects (HS) and chronic kidney disease patients in peritoneal dialysis (PD).

SFN inhibited HCV replication by upregulating Nrf2 expression.

8 images

(9 references)

产品编号 ABIN2855612

发货至: 中国

Datasheet as PDF

抗原 See all NRF2 (NFE2L2) 抗体

NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))
抗原表位
22

15

9

8

8

7

7

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Internal Region
适用
120

57

54

5

4

3

2

2

1

1

1

1

1

1
人, 大鼠
宿主
113

9

1
兔
Compatible Secondaries
克隆类型
104

19
多克隆
标记
58

10

7

6

4

4

4

4

4

4

4

4

1

1

1

1

1

1

1

1

1

1
This NRF2 antibody is un-conjugated
应用范围
96

52

31

29

20

18

15

14

14

9

7

2

2

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)

产品特性

Rabbit Polyclonal antibody to NRF2 (nuclear factor (erythroid-derived 2)-like 2)
NRF2 antibody [N2C2], Internal

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary.

亚型

IgG

Top Product

Discover our top product NFE2L2 Primary Antibody
anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (pSer40) antibody
NFE2L2 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (AA 430-480) antibody
NFE2L2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (AA 366-605) antibody
NFE2L2 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (AA 382-604) antibody
NFE2L2 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (acLys599) antibody
NFE2L2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) antibody
NFE2L2 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (N-Term) antibody
NFE2L2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (AA 356-589) antibody
NFE2L2 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 1A6G6 unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (pSer40) antibody
NFE2L2 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS, IF (cc) 宿主: 兔 Monoclonal 7G4 unconjugated

anti-Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2) (AA 71-170) antibody
NFE2L2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1B8 unconjugated

应用备注

Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

说明

Positive Control: MDA-MB-231 NE , MDA-MB-231 NE treated tBHQ , 3xFlag-human NFE2L2-transfected 293T

限制

仅限研究用
状态

Liquid

浓度

0.51 mg/mL

缓冲液

1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Tsai, Chen, Lin, Chou, Chen, Ho, Lin, Hwang: "Miconazole Contributes to NRF2 Activation by Noncanonical P62-KEAP1 Pathway in Bladder Cancer Cells." in: Drug design, development and therapy, Vol. 14, pp. 1209-1218, (2020) (PubMed).

Batubara, Astuti, Prastya, Ilmiawati, Maeda, Suzuki, Hamamoto, Takemori: "The Antiaging Effect of Active Fractions and Ent-11α-Hydroxy-15-Oxo-Kaur-16-En-19-Oic Acid Isolated from Adenostemma lavenia (L.) O. Kuntze at the Cellular Level." in: Antioxidants (Basel, Switzerland), Vol. 9, Issue 8, (2020) (PubMed).

Chou, Lin, Dzhagalov, Chen, Lin, Lin, Chen, Chen, Fu: "Vaccine adjuvant activity of a TLR4-activating synthetic glycolipid by promoting autophagy." in: Scientific reports, Vol. 10, Issue 1, pp. 8422, (2020) (PubMed).

Neitzel, Seiwert, Göder, Diehl, Weber, Nagel, Stroh, Rasenberger, Christmann, Fahrer: "Lipoic Acid Synergizes with Antineoplastic Drugs in Colorectal Cancer by Targeting p53 for Proteasomal Degradation." in: Cells, Vol. 8, Issue 8, (2020) (PubMed).

Luo, Shang, Brooks, Jiagge, Zhu, Buschhaus, Conley, Fath, Davis, Gheordunescu, Wang, Harouaka, Lozier, Triner, McDermott, Merajver, Luker, Spitz, Wicha: "Targeting Breast Cancer Stem Cell State Equilibrium through Modulation of Redox Signaling." in: Cell metabolism, Vol. 28, Issue 1, pp. 69-86.e6, (2019) (PubMed).

García Trejo, Arellano Buendía, Sánchez Reyes, García Arroyo, Arguello García, Loredo Mendoza, Tapia, Sánchez Lozada, Osorio Alonso: "The Beneficial Effects of Allicin in Chronic Kidney Disease Are Comparable to Losartan." in: International journal of molecular sciences, Vol. 18, Issue 9, (2018) (PubMed).

Fragoulis, Siegl, Fendt, Jansen, Soppa, Brandenburg, Pufe, Weis, Wruck: "Oral administration of methysticin improves cognitive deficits in a mouse model of Alzheimer's disease." in: Redox biology, Vol. 12, pp. 843-853, (2018) (PubMed).

García-Trejo, Arellano-Buendía, Argüello-García, Loredo-Mendoza, García-Arroyo, Arellano-Mendoza, Castillo-Hernández, Guevara-Balcázar, Tapia, Sánchez-Lozada, Osorio-Alonso: "Effects of Allicin on Hypertension and Cardiac Function in Chronic Kidney Disease." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 3850402, (2017) (PubMed).

Arellano-Buendía, Tostado-González, García-Arroyo, Cristóbal-García, Loredo-Mendoza, Tapia, Sánchez-Lozada, Osorio-Alonso: "Anti-Inflammatory Therapy Modulates Nrf2-Keap1 in Kidney from Rats with Diabetes." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 4693801, (2017) (PubMed).
抗原

NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

别名

NRF2 (NFE2L2 产品)

别名

Nrf2 antibody, nfe2l2 antibody, wu:fc15g09 antibody, wu:fj67e03 antibody, NF-E2R2 antibody, NRF2 antibody, AI194320 antibody, nuclear factor, erythroid 2-like 2a antibody, nuclear factor, erythroid 2 like 2 S homeolog antibody, nuclear factor, erythroid 2 like 2 antibody, nuclear factor, erythroid derived 2, like 2 antibody, nuclear factor, erythroid 2-like 2 antibody, nfe2l2a antibody, nfe2l2.S antibody, NFE2L2 antibody, Nfe2l2 antibody

背景

NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM]

Cellular Localization: Cytoplasm , cytosol , Nucleus

分子量

68 kDa110

基因ID

4780

途径

ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling