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POU2AF1 抗体 (C-Term)

This 山羊 多克隆 antibody specifically detects POU2AF1 in WB, IHC 和 ELISA. It exhibits reactivity toward 人.
产品编号 ABIN768553
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for POU2AF1 抗体 (C-Term) (ABIN768553)

抗原

See all POU2AF1 抗体
POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

适用

  • 100
  • 20
  • 5

宿主

  • 86
  • 30
  • 1
山羊

克隆类型

  • 64
  • 53
多克隆

标记

  • 38
  • 8
  • 6
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
This POU2AF1 antibody is un-conjugated

应用范围

  • 87
  • 59
  • 48
  • 27
  • 26
  • 26
  • 20
  • 15
  • 14
  • 12
  • 10
  • 6
  • 2
  • 2
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA

质量等级

Verified
  • 抗原表位

    • 24
    • 15
    • 15
    • 11
    • 6
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    C-Term

    原理

    POU2AF1

    序列

    DSDAYALNHT LSVE

    交叉反应

    Cow, 犬, 人

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-DSDAYALNHTLSVE, from the C Terminus of the protein sequence according to NP_006226.2.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells. Recommended concentration, 3-5 μg/mL.

    Western Blot: In transfected HEK293 transiently expressing full-length Human POU2AF1 (myc and DYKDDDDK tagged), a band of approx. 38 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag

    Peptide ELISA: antibody detection limit dilution 1:8000.

    说明

    Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

    别名

    POU2AF1

    背景

    POU2AF1, POU class 2 associating factor 1, BOB1, OBF-1, OBF1, OCAB, POU domain, class 2, associating factor 1

    分子量

    38kDa

    基因ID

    5450

    NCBI登录号

    NP_006226
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