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PRAS40 抗体 (pThr246)

This 兔 单克隆 antibody specifically detects PRAS40 in WB, IHC, ELISA, IF 和 IP. It exhibits reactivity toward 人, 小鼠 和 大鼠.
产品编号 ABIN8119640
发货至: 中国
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Quick Overview for PRAS40 抗体 (pThr246) (ABIN8119640)

抗原

See all PRAS40 (AKT1S1) 抗体
PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

适用

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人, 小鼠, 大鼠

宿主

  • 103
  • 14
兔

克隆类型

  • 62
  • 55
单克隆

标记

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This PRAS40 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

克隆位点

AB2207
  • 抗原表位

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    pThr246

    原理

    PRAS40 (Phospho Thr246) Rabbit mAb

    特异性

    The antibody detects endogenous levels of PRAS40 protein only when phosphorylated at Thr246. The antibody does not cross-react with PRAS40 phosphorylated at other sites.

    纯化方法

    The antibody was purified by protein A affinity chromatography.

    免疫原

    Recombinant protein (or fragment).

    亚型

    IgG
  • 应用备注

    WB, 1:2000-1:10000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid freeze / thaw cycles

    储存条件

    -20 °C

    储存方法

    Store at-20°C. Avoid freeze / thaw cycles.
  • 抗原

    PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

    别名

    PRAS40

    背景

    Synonyms: AKT1S1, PRAS40, Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate

    Background: AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al. , 2003 [PubMed 12524439]) . [supplied by OMIM, Mar 2008],

    Gene Name: AKT1S1

    Protein Name: PRAS40 (Phospho Thr246)

    基因ID

    84335

    UniProt

    Q96B36

    途径

    Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect
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