Melanoma gp100 抗体 (AA 376-502)
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Quick Overview for Melanoma gp100 抗体 (AA 376-502) (ABIN8093422)
抗原
See all Melanoma gp100 (PMEL) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 376-502
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原理
- PMEL17 / Melanoma gp100 Antibody
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免疫原
- A recombinant fragment (around amino acids 376-502) of human SILV protein (exact sequence is proprietary) was used as the immunogen for the gp100 Antibody for IHC/PMEL antibody.
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亚型
- IgG, kappa
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anti-Premelanosome Protein (PMEL) antibody
PMEL 适用: 人, 大鼠, 猴, 犬 IHC, WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 10E5 unconjugated
anti-Premelanosome Protein (PMEL) (AA 376-502) antibodyPMEL 适用: 人 IHC, WB, ELISA 宿主: 小鼠 Monoclonal PMEL-2037 unconjugated
anti-Premelanosome Protein (PMEL) (C-Term) antibodyVerified PMEL 适用: 人, 小鼠 WB, IF, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) antibodyPMEL 适用: 人 IHC, WB, FACS, IF 宿主: 小鼠 Monoclonal HMB45 unconjugated
anti-Premelanosome Protein (PMEL) (N-Term) antibodyPMEL 适用: 人, 大鼠, 小鼠, 马, 犬, Cow, 山羊, 豚鼠, 兔 IHC, WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) antibodyPMEL 适用: 人 IHC, WB 宿主: 兔 Monoclonal PMEL-1825R unconjugated Recombinant Antibody
anti-Premelanosome Protein (PMEL) antibodyPMEL 适用: 人, 大鼠, 小鼠 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) (C-Term) antibodyPMEL 适用: 人, 小鼠 IHC, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) (AA 25-467) antibodyPMEL 适用: 人 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) antibodyPMEL 适用: 人 IHC 宿主: 兔 Monoclonal 617R unconjugated Recombinant Antibody
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应用备注
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1. Optimal dilution of the gp100 Melanoma Marker/PMEL antibody should be determined by the researcher.
2. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- gp100 Melanoma Marker/PMEL antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.
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- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
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别名
- PMEL17 / Melanoma gp100
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背景
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Premelanosome protein (PMEL) is a melanocyte lineage-associated glycoprotein encoded by the PMEL gene and widely known in the literature as gp100 or Pmel17. This melanosome-associated protein is expressed in melanocytes and melanocytic tumors and serves as a well-established melanocyte lineage marker in dermatopathology. gp100 Antibody for IHC / PMEL Melanocyte Lineage Marker (clone MSVA-617R) is designed for immunohistochemistry detection of melanocytic cells in formalin-fixed paraffin-embedded tissue sections. In immunohistochemistry studies, gp100 staining highlights melanocytes and melanoma tumor cells, supporting evaluation of melanocytic differentiation in tissue specimens.
Immunohistochemistry analysis of PMEL typically demonstrates cytoplasmic staining within melanocytes located along the basal layer of the epidermis in normal skin. In melanoma tissue, gp100 immunohistochemistry staining is commonly observed in tumor cells forming nests or sheets within the lesion. Because gp100 expression is largely restricted to melanocytic lineage cells, gp100 Antibody for IHC is frequently used in melanoma pathology to help identify melanocytic tumors and distinguish melanoma from non-melanocytic malignancies in tissue sections.
Large-scale protein microarray screening of clone MSVA-617R evaluated antibody binding across thousands of human proteins and demonstrated selective recognition of PMEL relative to other proteins present on the array. Protein microarray specificity analysis therefore supports the selectivity of gp100 Antibody for IHC clone MSVA-617R for the intended PMEL target. Such specificity screening provides useful experimental evidence supporting antibody selectivity across a broad proteomic background.
Immunohistochemistry analysis using human tissue microarray (TMA) panels containing multiple normal and cancer tissues further demonstrates the melanocyte lineage specificity of gp100 staining. In tissue microarray studies, strong cytoplasmic staining is observed in melanoma cells and normal melanocytes, whereas most non-melanocytic tissues show little or no staining. Tissue microarray immunohistochemistry therefore reinforces the value of PMEL as a melanocyte lineage marker widely used in melanoma research and diagnostic pathology investigations.
gp100 Antibody for IHC / PMEL Melanocyte Lineage Marker clone MSVA-617R detects the gp100 protein in melanocytes and melanoma cells and supports investigation of melanocytic lineage markers in tissue specimens. The combination of immunohistochemistry staining patterns observed in tissue microarray studies together with protein microarray specificity data supports the use of this antibody for studies examining melanocyte biology, melanoma tumor tissues, and melanocytic differentiation in histological samples.
This antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues. -
UniProt
- P40967
抗原
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