Recombinant MSH2 抗体 (full length)
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北京 101111
Quick Overview for Recombinant MSH2 抗体 (full length) (ABIN8093331)
抗原
See all MSH2 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- full length
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原理
- MSH2 Antibody / MutS homolog 2
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免疫原
- Recombinant full-length human MSH2 protein was used as the immunogen for the MSH2 / MutS homolog 2 antibody.
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亚型
- IgG2a, kappa
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anti-Mismatch Repair Protein 2 (MSH2) (AA 327-427) antibody
MSH2 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, Hamster WB, IHC, IF, FACS 宿主: 小鼠 Monoclonal MSH2-2622 unconjugated
anti-Mismatch Repair Protein 2 (MSH2) (AA 442-586) antibodyMSH2 适用: 人, 小鼠 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 2E2C7 unconjugated
anti-Mismatch Repair Protein 2 (MSH2) (AA 1-300) antibodyMSH2 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Mismatch Repair Protein 2 (MSH2) (AA 337-583) antibodyMSH2 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mismatch Repair Protein 2 (MSH2) (AA 1-934) antibodyMSH2 适用: 人 WB, IF, PLA 宿主: 兔 Polyclonal unconjugated
anti-Mismatch Repair Protein 2 (MSH2) (AA 337-583) antibodyMSH2 适用: 人 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal 6B4F7 unconjugated
anti-Mismatch Repair Protein 2 (MSH2) (AA 224-313) antibodyMSH2 适用: 人, 犬 IHC 宿主: 小鼠 Monoclonal SYP-3551 unconjugated
anti-Mismatch Repair Protein 2 (MSH2) (C-Term) antibodyMSH2 适用: 人, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Mismatch Repair Protein 2 (MSH2) (AA 442-586) antibodyMSH2 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 4F10D6 unconjugated
anti-Mismatch Repair Protein 2 (MSH2) (AA 835-934) antibodyMSH2 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 4B2 unconjugated
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应用备注
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1. Optimal dilution of the MSH2 Antibody for IHC / MutS homolog 2 Immunohistochemistry Antibody should be determined by the researcher.
2. This MSH2 / MutS homolog 2 antibody is recombinantly produced by expression in CHO cells.
3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Antibody in 1X PBS with 0.05 % BSA, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- MSH2 / MutS homolog 2 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.
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- MSH2 (Mismatch Repair Protein 2 (MSH2))
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别名
- MSH2
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背景
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MutS homolog 2 (MSH2) is a nuclear DNA mismatch repair protein encoded by the MSH2 gene and is a central component of the cellular machinery responsible for maintaining genomic stability. MSH2 Antibody for IHC is designed for immunohistochemical detection of MutS homolog 2 / MSH2 in tissue sections, enabling visualization of nuclear expression patterns associated with DNA mismatch repair activity. MSH2 forms heterodimeric complexes with other mismatch repair proteins, including MSH6 and MSH3, allowing recognition and correction of base-base mismatches and insertion-deletion loops that occur during DNA replication. Because this repair pathway is essential for preserving genomic integrity, MSH2 expression is widely studied in cancer biology and tumor diagnostics.
MSH2 antibody, also referred to as MutS homolog 2 antibody or hMSH2 antibody in the literature, detects a nuclear protein that is broadly expressed in proliferating epithelial tissues and lymphoid cells. In immunohistochemistry applications, MSH2 staining typically appears as distinct nuclear labeling in cells with intact mismatch repair activity. MSH2 Antibody for IHC is therefore particularly useful for evaluating DNA mismatch repair status in tissue specimens, as loss of nuclear staining in tumor cells may indicate mismatch repair deficiency while surrounding non-neoplastic cells retain nuclear positivity and serve as internal controls.
Immunohistochemistry analysis using MSH2 Antibody for IHC plays a critical role in research and pathology studies examining mismatch repair deficiency and microsatellite instability. Loss of MSH2 expression has been strongly associated with hereditary cancer syndromes such as Lynch syndrome as well as a subset of sporadic colorectal, endometrial, and other carcinomas. Because of this biological significance, detection of MSH2 protein expression in formalin-fixed paraffin-embedded tissues is widely used to investigate tumor biology, genomic instability, and DNA repair mechanisms.
MSH2 Antibody for IHC (clone MSVA-902M) has been tested on many normal and cancer tissues, demonstrating nuclear immunohistochemical staining patterns that align with expression data reported in the Human Protein Atlas. This recombinant mouse monoclonal antibody provides a reliable reagent for immunohistochemistry studies evaluating mismatch repair protein expression, nuclear DNA repair pathways, and tumor-associated alterations in MSH2 expression.
This antibody is also part of a broader collection of < a href='/ihc-antibodies-tissue-microarray-validated.html'>IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues. -
途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
抗原
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