AKT 1/2/3 抗体 (pTyr312, pTyr315, pTyr316) (PE-Cy5)
Our Local Distributor
北京 101111
Quick Overview for AKT 1/2/3 抗体 (pTyr312, pTyr315, pTyr316) (PE-Cy5) (ABIN756229)
抗原
适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- pTyr312, pTyr315, pTyr316
-
特异性
- This antibody will detect AKT 1 when phosphorylated at Tyr315 in Human, Mouse, Rat or Bovine, AKT2 when phosphorylated at Tyr316 in Human, Mouse, Rat, or Bovine, and AKT3 when phosphorylated at Tyr312 in Human, Mouse, Rat, or Bovine.
-
交叉反应
- 人, 小鼠, 大鼠
-
预测反应
- Dog,Cow,Sheep,Pig,Chicken,Rabbit
-
纯化方法
- Purified by Protein A.
-
免疫原
- KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Tyr315
-
亚型
- IgG
-
-
anti-AKT 1/2/3 (AA 401-479) antibody
适用: 人, 小鼠, 大鼠, Cow, Pig WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pSer473) antibody适用: 人, 小鼠, 大鼠, Cow, 兔, 小鸡, 鱼 WB, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pSer472) antibody适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 antibody适用: 人, 小鼠, 大鼠, Chimpanzee WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 14E5-16C8-25F6 unconjugated
anti-AKT 1/2/3 (pTyr474) antibody适用: 人, 小鼠 WB, FACS, IF (cc), IF (p), ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pTyr312), (pTyr315), (pTyr316) antibody适用: 人, 小鼠, 大鼠 WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pTyr312), (pTyr315), (pTyr316) antibody适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 (pThr305), (pThr308), (pThr309) antibody适用: 人, 小鼠, 大鼠 WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-AKT 1/2/3 antibody适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB3180 unconjugated Recombinant Antibody
anti-AKT 1/2/3 antibody适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB3185 unconjugated Recombinant Antibody
-
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 μg/μL
-
缓冲液
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
-
有效期
- 12 months
-
-
- AKT 1/2/3
-
别名
- AKT1+2+3
-
背景
-
Synonyms: AKT1/AKT2/AKT3 Tyr315+Tyr316+Tyr312, AKT1/AKT2/AKT3Tyr315/316/312, AKT1/2/3 phospho Y315 + Y316 + Y312, AKT 1, AKT, AKT1, AKT-1, AKT1_HUMAN, AKT 2, AKT2, AKT-2, AKT2_HUMAN, AKT 3, AKT3, AKT-3, AKT3_HUMAN, C AKT, cAKT, MGC9965, MGC99656, Oncogene AKT1, PKB, PKB alpha, PKB-ALPHA, PKB beta, PKB gamma, PRKBA, Protein Kinase B Alpha, Protein kinase B, Proto-oncogene c-Akt, RAC Alpha, RAC alpha serine/threonine protein kinase, RAC, RAC PK Alpha, Rac protein kinase alpha, RAC Serine/Threonine Protein Kinase, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha, v akt murine thymoma viral oncogene homolog 1, vAKT Murine Thymoma Viral Oncogene Homolog 1.
Background: AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82 % identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold, overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.
-
分子量
- 56kDa
-
基因ID
- 207, 208, 10000
抗原
-
